• 主题
高级搜索  >
历史搜索 清空历史
首页> 外国专利> WAYS OF OBTAINING A NEW GENERATION OF SAFE WITH THE BIOLOGICAL POINT OF VIEW OF KLH PRODUCTS USED FOR CANCER TREATMENT, TO DEVELOP CONCURNED THERAPEUTIC VACCINES AND AS A PROMOTION OF ACTUATED THERAPEUTIC VACCINES AND IN THE QUALITY OF STIMULATING THERAPY.

WAYS OF OBTAINING A NEW GENERATION OF SAFE WITH THE BIOLOGICAL POINT OF VIEW OF KLH PRODUCTS USED FOR CANCER TREATMENT, TO DEVELOP CONCURNED THERAPEUTIC VACCINES AND AS A PROMOTION OF ACTUATED THERAPEUTIC VACCINES AND IN THE QUALITY OF STIMULATING THERAPY.

机译：从生物学观点出发，从用于癌症治疗的KLH产品中获得新的安全来源，以开发持续的治疗性疫苗，并促进治疗性疫苗的产生和提高治疗的质量。

页面导航
  • 摘要
  • 著录项
  • 相似文献

摘要

FIELD: biochemistry.;SUBSTANCE: method for the isolation and/or purification of hemocyanin is described, which includes a) providing the composition of hemocyanin, obtained from marine mollusk, the specified composition is a serum hemolymph; b) reducing conductivity of the composition provided in a) stage to a value of 30 mS/cm to 10 mS/cm by adding dilution buffer; c) applying a diluted formulation containing hemocyanin obtained in b) stage onto an anion exchange chromatography column; d) rinsing said column with hemocyanin purification buffer, preferably to remove salts and other proteins; e) and eluting hemocyanin from said column by adding a second buffer. In addition, a method for the isolation and/or purification of an immunocyanin is presented, including: a) providing the composition of hemocyanin, obtained from marine mollusk, the specified composition is a serum hemolymph; b) reducing conductivity of the composition provided in a) stage to a value of 30 mS/cm to 10 mS/cm by adding dilution buffer; c) applying a diluted formulation containing hemocyanin obtained in b) stage onto an anion exchange chromatography column; d) rinsing said column with hemocyanin purification buffer, preferably to remove salts and other proteins; e) dissociating hemocyanin into subunits by adding dissociation buffer with pH from 8 to 10, not containing Ca++ and Mg++ ions; and f) eluting the immunocyanin from said column by adding a second buffer. Use of an anion exchange chromatography column is also disclosed containing a matrix material with a particle size of more than 30 mcm and a pore size of 3,000 to 8,000 A (Angstrem) for purifying hemocyanin or subunits thereof from hemolymph serum.;EFFECT: invention allows to isolate and/or purify hemocyanin and subunits thereof using anion-exchange chromatography.;12 cl, 6 ex
机译：领域：描述了用于分离和/或纯化血蓝蛋白的方法，其包括：a）提供从海洋软体动物获得的血蓝蛋白的组成，指定的组成是血清血淋巴; b）通过添加稀释缓冲液将a）阶段中提供的组合物的电导率降低至30mS / cm至10mS / cm的值; c）将在b）阶段获得的含有血蓝蛋白的稀释制剂施加到阴离子交换色谱柱上; d）用血蓝蛋白纯化缓冲液冲洗所述柱，优选除去盐和其他蛋白质; e）并通过添加第二缓冲液从所述柱洗脱血蓝蛋白。另外，提出了一种分离和/或纯化免疫花青素的方法，包括：a）提供从海洋软体动物获得的血青素的组合物，指定的组合物是血清血淋巴; b）通过添加稀释缓冲液将a）阶段中提供的组合物的电导率降低至30mS / cm至10mS / cm的值; c）将在b）阶段获得的含有血蓝蛋白的稀释制剂施加到阴离子交换色谱柱上; d）用血蓝蛋白纯化缓冲液冲洗所述柱，优选除去盐和其他蛋白质; e）通过加入pH 8至10的不含Ca ++ 和Mg ++ 离子的解离缓冲液将血蓝蛋白分解为亚基; f）通过添加第二缓冲液从所述柱洗脱免疫花青素。还公开了使用阴离子交换色谱柱，该色谱柱包含粒径大于30 mcm，孔径为3,000至8,000 A的基质材料（Angstrem），用于从血淋巴血清中纯化血蓝蛋白或其亚基。使用阴离子交换色谱法分离和/或纯化血蓝蛋白及其亚基。; 12 cl，6 ex

著录项

相似文献

  • 专利
  • 外文文献
获取专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号