首页> 外国专利> ARTIFICIAL YkuJ-MPER GENE ENCODING YkuJ-MPER RECOMBINANT PROTEIN-IMMUNOGEN, pET21a-YkuJ-MPER RECOMBINANT PLASMID DNA, PROVIDING EXPRESSION OF ARTIFICIAL YkuJ-MPER GENE, AND YkuJ-MPER RECOMBINANT PROTEIN-IMMUNOGEN, WHICH IS CARRIER OF MEMBRANE-PROXIMAL AREA OF HIV-1 AND INTENDED FOR INDUCTION IN BROADLY NEUTRALIZING ANTIBODIES

ARTIFICIAL YkuJ-MPER GENE ENCODING YkuJ-MPER RECOMBINANT PROTEIN-IMMUNOGEN, pET21a-YkuJ-MPER RECOMBINANT PLASMID DNA, PROVIDING EXPRESSION OF ARTIFICIAL YkuJ-MPER GENE, AND YkuJ-MPER RECOMBINANT PROTEIN-IMMUNOGEN, WHICH IS CARRIER OF MEMBRANE-PROXIMAL AREA OF HIV-1 AND INTENDED FOR INDUCTION IN BROADLY NEUTRALIZING ANTIBODIES

机译：包含YkuJ-Mper重组蛋白-免疫原，pET21a-YkuJ-Mper重组质体DNA的人工YkuJ-Mper基因，提供人工YkuJ-Mper基因的表达，和Wku-Mper重组蛋白-免疫原的抗原性抗原。 HIV-1，旨在诱导广泛中和的抗体。

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FIELD: biotechnology.;SUBSTANCE: group of inventions relates to the field of biotechnology, genetic engineering and medicine. Proposed artificial gene YkuJ-MPER encodes a protein-immunogen YkuJ-MPER against HIV-1 with the nucleotide sequence of SEQ ID NO:1 of length of 360 bps. Recombinant plasmid DNA of pET21a-YkuJ-MPER type is also proposed, designed to produce a protein-immunogen that induces HIV-specific B-cell response, with a size of 5,700 bps and a molecular weight of 3.8·10³ kDa containing the YkuJ-MPER gene, encoding the YkuJ-MPER anti-HIV-1 protein-immunogen, under the control of the RNA polymerase promoter of phage T7, ensuring its expression in E. coli cells. Artificial YkuJ-MPER immunogen protein is also proposed, consisting of a B. subtilis YkuJ protein site and two HIV-1 MPER sites, having a molecular weight of 14.2 kDa and the amino acid sequence of SEQ ID NO:2.;EFFECT: group of inventions provides an effective induction of broadly neutralizing anti-HIV-1 antibodies in the selection of the optimal immunization strategy.;3 cl, 12 dwg, 1 tbl, 6 ex

机译：领域：生物技术;发明领域：一组发明涉及生物技术，基因工程和医学领域。拟议的人工基因YkuJ-MPER编码一种针对HIV-1的蛋白质免疫原YkuJ-MPER，其核苷酸序列SEQ ID NO：1的长度为360 bps。还提出了pET21a-YkuJ-MPER类型的重组质粒DNA，旨在产生一种蛋白免疫原，该蛋白免疫原可诱导HIV特异性B细胞反应，大小为5700 bps，分子量为3.8·10 ^{3 < / Sup> kDa含有在噬菌体T7的RNA聚合酶启动子的控制下编码YkuJ-MPER抗HIV-1蛋白免疫原的YkuJ-MPER基因，确保其在大肠杆菌细胞中表达。还提出了人工YkuJ-MPER免疫原蛋白，它由枯草芽孢杆菌YkuJ蛋白位点和两个HIV-1 MPER位点组成，分子量为14.2kDa，氨基酸序列为SEQ ID NO：2。的发明提供了在选择最佳免疫策略时有效诱导广泛中和的抗HIV-1抗体的方法。3cl，12 dwg，1 tbl，6 ex}

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公开/公告号RU2679076C1

专利类型
公开/公告日2019-02-05

原文格式PDF
申请/专利权人 FEDERALNOE BYUDZHETNOE UCHREZHDENIE NAUKI GOSUDARSTVENNYJ NAUCHNYJ TSENTR VIRUSOLOGII I BIOTEKHNOLOGII VEKTOR FEDERALNOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIYA CHELOVEKA (FBUN GNTS VB VEKTOR ROSPOTREBNADZORA);
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申请/专利号RU20170139042
发明设计人 RUDOMETOV ANDREJ PAVLOVICH (RU);ANDREEVA NADEZHDA BORISOVNA (RU);SHCHERBAKOV DMITRIJ NIKOLAEVICH (RU);KARPENKO LARISA IVANOVNA (RU);BAKULINA ANASTASIYA YUREVNA (RU);
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申请日2017-11-09
分类号C12N15/62;C12N15/70;C07K14/16;C07K14/195;
国家 RU
入库时间 2022-08-21 11:46:25

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