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A method for the treatment of osteoarthritis of the knee
A method for the treatment of osteoarthritis of the knee
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机译：一种治疗膝骨关节炎的方法
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摘要
FIELD: medicine.SUBSTANCE: invention relates to traumatology and orthopaedics, and can be used for treating osteoarthrosis of knee joint. Introduced into an affected joint through a lower-lateral penetration needle 20G (0.9 × 70 mm) into the external cavity of knee joint of 4.5 ml suspension of stromal-vascular fraction of cell product in Hartman's solution. A cellular product is represented by a suspension of stromal-vascular fraction cells prepared by enzymatic treatment of adipose tissue in volume of 100–300 ml, taken by syringe tumescent liposuction. Enzymatic treatment of washed fatty tissue in Hartmann solution is carried out with 0.15 % collagenase of type 2. Further incubation is carried out on shaker for 30 minutes at temperature of 37 °C. Mixture is filtered through a sieve with pore diameter of 70–100 mcm. It is followed by washing in Hartmann's solution 3 times. It is centrifuged for 7 minutes at 300 g.EFFECT: method provides higher stable therapeutic effect due to introduction of regenerative cells participating in cartilage tissue repair within one surgical procedure.1 cl, 2 ex
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机译：技术领域本发明涉及创伤学和骨科，并且可以用于治疗膝关节的骨关节炎。通过下侧穿刺针20G（0.9×70 mm）引入受影响的关节中，将哈特曼溶液中的细胞产物基质血管部分的4.5 ml悬浮液注入膝关节的外腔。细胞产物的代表是间质-血管级分细胞的悬浮液，该悬浮液是通过酶促处理脂肪组织（通过注射器肿胀吸脂术）制成的脂肪组织，体积为100-300 ml。用0.1％的2型胶原酶在Hartmann溶液中对洗涤过的脂肪组织进行酶处理。进一步在37°C的振荡器上孵育30分钟。通过孔径为70–100 mcm的筛子过滤混合物。随后在Hartmann溶液中洗涤3次。以300 g的速度离心7分钟。效果：由于在一种外科手术中引入了参与软骨组织修复的再生细胞，该方法提供了更高的稳定治疗效果。1cl，2 ex
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