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PCR Lysinibacillus fusiformis Method for detecting Lysinibacillus fusiformis a suspicious pathogen of Bombus terrestris using ultra-rapid Polymerase Chain Reaction technique
PCR Lysinibacillus fusiformis Method for detecting Lysinibacillus fusiformis a suspicious pathogen of Bombus terrestris using ultra-rapid Polymerase Chain Reaction technique
Lysinibacillus suspected pathogen for suspicion of Western dune using ultrafast PCR A method for detecting of fusiformis is disclosed . According to a detection method according to an embodiment of the present invention, the presence of Lysinibacillus fusiformis is detected by performing an ultrafast PCR process with a primer composition comprising primer pairs of SEQ ID NOs: 1 and 2. In particular, Lysinibacillus fusiformis to optimize the conditions for specific genes high-speed PCR process, to minimize the time it takes to Lysinibacillus fusiformis detected by Western common carder bee adult suspected to be infected with Lysinibacillus fusiformis. By applying such optimized Lysinibacillus fusiformis- specific ultrafast PCR, infection of Lysinibacillus fusiformis can be determined in 4 minutes and 22 seconds based on the number of 1.0 × 0 8 molecules, and quantitatively measuring recombinant DNA of at least 1.0 × 0 1 molecule. can do. In addition, detection of pathogens using suspicion of infection caused by Lysinibacillusfusiformis- specific gene ultrafast PCR showed a positive response, which is useful for quantitative detection in the field as well as in the laboratory.
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机译：使用超快速PCR A 方法检测疑似镰孢菌的I.Lysinibacillus 疑似病原体 Dune 。根据本发明的一个实施方案的检测方法，通过用包含SEQ ID NO：1和2的引物对的引物组合物进行超快速PCR过程来检测 Lysinibacillus fusiformis 的存在。尤其是，镰孢杆菌可优化特定基因的高速PCR过程的条件，从而最大程度地减少怀疑可疑的西方普通梳毛蜂成虫感染镰孢杆菌的时间。通过应用这种优化的镰孢镰刀菌-特异性超快PCR，可以感染镰孢杆菌。根据1.0×0 8 分子的数量，在4分22秒内确定，并定量测量至少1.0×0 1 分子的重组DNA。可以做。此外，利用怀疑的 Lysinibacillus fusiformis-特异性基因超快PCR检测病原体表现出了积极的反应，这对于现场定量检测以及在实验室里。
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