20- Method for producing transgenic plant with increased content of 20-hydroxyecdysone using genes from insect and the plant thereof

• 1. 利用源自菠菜的CYP85基因的20-羟基蜕皮激素含量增加的转化植物体的制备方法 [P] . 中国专利： CN106102451B . 2019.08.09
• 2. 20-羟基蜕皮激素在促进冬虫夏草菌芽生孢子发育形成菌丝中的应用 [P] . 中国专利： CN110106092B . 2021.03.12
• 3. 20- Method for producing transgenic plant with increased content of 20-hydroxyecdysone using genes from insect and the plant thereof [P] . 韩国专利： KR102107412B1 . 2020-05-07

  机译： 20-使用来自昆虫的基因及其植物生产具有增加的20-羟基蜕皮酮含量的转基因植物的方法

• 4. Method for producing transgenic plant with increased content of 20-hydroxyecdysone using genes from insect and the plant thereof [P] . 韩国专利： KR1021074120000B1 . 2020.05.07
• 5. METHOD FOR PRODUCING TRANSGENIC PLANT HAVING INCREASED CONTENT OF 20-HYDROXYECDYSONE USING INSECT-DERIVED GENE AND PLANT PRODUCED BY THE SAME [P] . 美国专利： US2020299720A1 . 2020-09-24

  机译： 利用昆虫源基因和由该植物生产的植物生产具有20-羟基乙二酮含量更高的转基因植物的方法

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• 2. High plant density increases seed Bt endotoxin content in Bt transgenic cotton [J] . CHEN Yuan, LIU Zhen-yu, HENG Li, 农业科学学报：英文版 . 2021,第007期
• 3. High plant density increases seed Bt endotoxin content in Bt transgenic cotton [J] . CHEN Yuan, LIU Zhen-yu, HENG Li, 农业科学学报（英文版） . 2021,第007期
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• 1. Responses of Transgenic Tobacco Plants with Increased Proline Content to Drought and/or Heat Stress [J] . Jana Pospisilova ,Daniel Haisel ,Radomira Vankova . 美国植物学期刊（英文） . 2011,第3期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. 用高效液相色谱测定20-羟基蜕皮酮滴度方法的改进 [C] . 钱明惠 ,范军祥 ,黄焕华 . 中国昆虫学会第八次全国会员代表大会暨2007年学术年会 . 2007
• 4. 多巴胺/蜕皮激素受体通过结合20-羟基蜕皮酮调节昆虫的变态发育 [A] . 张俊英 . 2018