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miRNA Method for Culturing Cornea Epithelial Cell by Inducing Differentiation of Induced Pluripotent Stem Cell Using miRNA and System for the Same
miRNA Method for Culturing Cornea Epithelial Cell by Inducing Differentiation of Induced Pluripotent Stem Cell Using miRNA and System for the Same
The present invention relates to a method for inducing differentiation of inducible pluripotent stem cells to cultivate corneal epithelial cells, which method comprises culturing the corneal epithelial cells containing DMEM F12, L-ascorbic acid, sodium selenite, Preparing a basic medium; Adding an inducer for culturing an inducible pluripotent stem cell to the basal medium; Preparing a feeder-free culture medium by coating the basic medium with the vitronectin recombinant human protein; Adding the inducer pluripotent stem cells to the feeder-containing medium; Culturing the induced pluripotent stem cells cultured in the feeder-containing medium in a differentiation medium; Adding the BMP4 to the differentiation medium to differentiate the cultured induced pluripotent stem cells into corneal stem cells; A step of sequentially adding Wnt3a and miRNA or differentiation of Wnt3a and miRNA to differentiate into corneal epithelial cells after the step of adding BMP4 to the differentiation medium; And culturing the cells differentiated from the induced pluripotent stem cells in a PI culture medium.
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