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MUTANT ALCOHOL OXIDASES AND USE THEREOF IN THE CONVERSION OF DIOLS AND POLYOLS
MUTANT ALCOHOL OXIDASES AND USE THEREOF IN THE CONVERSION OF DIOLS AND POLYOLS
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机译：突变型醇氧化酶及其在转化二元醇和多元醇中的用途
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摘要
The invention relates to the field of enzyme engineering and biocatalysis, in particular to mutated alcohol oxidases (AOXs) and their application in the biocatalytic conversion of diols and polyols. Provided is mutant AOX enzyme having glycerol oxidase activity, said mutant AOX showing at least 50% sequence identity to the amino acid sequence of AOX from P. chrysosporium (PcAOX; SEQ ID NO:l), Gloeophyllum trabeum (GtAOX; SEQ ID NO:2), Candida, boidinii (CbAOX; SEQ ID NO:3), Pichia pastoris (PpAOX; SEQ ID NO:4), or Pichia angusta (PaAOX; SEQ ID NO:5), and comprising one or more of the following mutations: (i) the amino acid corresponding to Phe at position 101 of PcAOX is mutated to Gin, Asn, His or Ser; (ii) the amino acid corresponding to Met at position 103 of PcAOX is mutated to Ser, Ala, Gly, Pro or Cys; (iii) the amino acid corresponding to Leu at position 317 of PcAOX is mutated to Val, He, Ala or Met; (iv) the amino acid corresponding to Tyr at position 407 of PcAOX is mutated to Phe, Val, Leu, He or Met; (v) the amino acid corresponding to Trp at position 560 of PcAOX is mutated to Cys, Met, Ala, Val, Leu or Ile.
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机译：本发明涉及酶工程和生物催化领域，尤其涉及突变的醇氧化酶（AOXs）及其在二醇和多元醇的生物催化转化中的应用。提供了具有甘油氧化酶活性的突变体AOX酶，所述突变体AOX与来自金孢假单胞菌（PcAOX; SEQ ID NO：1）的AOX的氨基酸序列显示至少50％的序列同一性， （GtAOX; SEQ ID NO：2），Candida，boidinii （CbAOX; SEQ ID NO：3），巴斯德毕赤酵母（PpAOX; SEQ ID NO：4）或安氏毕赤酵母（PaAOX; SEQ ID NO：5），并包含一个或多个以下突变：（i）对应于PcAOX 101位Phe的氨基酸突变为Gin，Asn，他或Ser; （ii）将对应于PcAOX的103位的Met的氨基酸突变为Ser，Ala，Gly，Pro或Cys; （iii）将对应于PcAOX 317位亮氨酸的氨基酸突变为Val，He，Ala或Met; （iv）将对应于PcAOX的407位上的Tyr的氨基酸突变为Phe，Val，Leu，He或Met; （v）对应于PcAOX的560位的Trp的氨基酸突变为Cys，Met，Ala，Val，Leu或Ile。
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