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RAPID DETECTION OF ANTIMICROBIAL RESISTANCE BY MICROBIAL RIBOSOME IMMUNOPRECIPITATION
RAPID DETECTION OF ANTIMICROBIAL RESISTANCE BY MICROBIAL RIBOSOME IMMUNOPRECIPITATION
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机译：微生物核糖体免疫沉淀法快速检测抗药性。
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摘要
The present invention provides a method for determining the resistance of a microorganism to a drug by detecting at least a transcript of a drug resistance gene from a microorganism in a biological sample, the method comprising the steps of: (i) lysing the cells by means of a chemical or a mechanical method, thereby obtaining a lysate and cell debris; (ii) obtaining a ribosome-antibody complex from the lysate using an antibody or a fragment thereof which binds specifically to a microorganism-ribosomal protein; (iii) purifying the mRNA associated to the ribosome-antibody complex by means of a nucleic acid extraction method; and (iv) submitting the resulting mRNA to a specific gene detection method, thereby identifying the at least one drug resistance gene transcript of the biological sample. Provided methods and kits allow determining the resistance to antibiotics of a biological sample in a rapid and reliable manner, thereby minimizing the risk of AMR and allowing the definition of the therapeutic potential of a selected antibiotic
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