A biological cartridge, a biological cartridge assembly, a microsphere preparation device, a shell assembling device, a biobrick preparation instrument, a biological ink preparation instrument, and a biological ink preparation system. A biological cartridge (131) comprises: a cartridge housing (1311), having a cavity; an isolation wall (1312), provided in the cavity, and dividing the cavity into a first accommodating cavity (1313) and a second accommodating cavity (1314) isolated from each other; a first cartridge inlet (13151) and a first cartridge outlet (13153), provided on the cartridge housing (1311), and communicated with the first accommodating cavity (1313) and fluidly isolated from the second accommodating cavity (1314); a second cartridge inlet (13152) and a second cartridge outlet (13154), provided on the cartridge housing (1311), and communicated with the second accommodating cavity (1314); and an internal flow channel (1316), located in the second accommodating cavity (1314), and communicated with the first accommodating cavity (1313) and the first cartridge outlet (13153).
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