A point-of-care diagnostic system comprising a method and related device is provided for detecting and/or quantitating pathogens and/or antimicrobial resistance in a sample, such as a bodily fluid. In various embodiments, the described method comprises Recombinase Polymerase Assay (RPA) using specifically designed primer sets and probes. In various embodiments, the described system comprises a detection module, and a data analysis and processing module, where the detection module comprises a fluid handling system for performing Recombinase Polymerase Assay (RPA) on the sample.
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