首页> 外国专利> PREPARATION METHOD FOR L-ALANYL-L-GLUTAMINE, ENZYME FOR L-ALANYL-L-GLUTAMINE PREPARATION, AND APPLICATION

PREPARATION METHOD FOR L-ALANYL-L-GLUTAMINE, ENZYME FOR L-ALANYL-L-GLUTAMINE PREPARATION, AND APPLICATION

机译:L-丙氨酰-L-谷氨酰胺的制备方法,制备L-丙氨酰-L-谷氨酰胺的酶及其应用

摘要

Provided is a preparation method for L-alanyl-L-glutamine, said method comprising: in the presence of α-amino acid ester acyltransferase, using L-alanine methyl ester hydrochloride and L-glutamine as substrates to prepare a reaction solution, adjusting the pH of the reaction solution to 7.0-9.0, and after reacting at a constant temperature of 20-40°C, collecting L-alanyl-L-glutamine. The α-amino acid ester acyltransferase is derived from elizabethkingia meningoseptica, and the amino acid sequence of the α-amino acid ester acyltransferase comprises the amino acid sequence in any one of SEQ ID NO: 1 – SEQ ID NO: 6. The preparation method is highly effective, simple, low-cost and environmentally friendly, has a high conversion rate, and may be widely applied in industrial scale production. Also provided are an enzyme for L-alanyl-L-glutamine preparation, and an application.
机译:提供了一种L-丙氨酰-L-谷氨酰胺的制备方法,所述方法包括:在α-氨基酸酯酰基转移酶的存在下,以L-丙氨酸甲酯盐酸盐和L-谷氨酰胺为底物制备反应液,调节其浓度。将反应溶液的pH调至7.0-9.0,并在20-40℃的恒定温度下反应后,收集L-丙氨酰-L-谷氨酰胺。 α-氨基酸酯酰基转移酶来源于脑膜炎沙门氏菌,α-氨基酸酯酰基转移酶的氨基酸序列包含SEQ ID NO:1〜SEQ ID NO:6中任一项的氨基酸序列。高效,简单,低成本,环保,转化率高,可广泛应用于工业规模生产。还提供了用于L-丙氨酰基-L-谷氨酰胺制备的酶及其应用。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号