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MARKERS OF ENDOPLASMIC RETICULUM STRESS IN OVARIAN FOLLICULAR FLUID PREDICT IVF SUCCESS

机译:卵巢卵泡预测IVF成功中的网状内网应激标记

摘要

The present invention discloses an unbiased "biomarker" method for the prediction of pregnancy success that is detectable in patient follicular fluid (FF) collected during egg retrieval in IVF procedures. Proteins that function in the cellular response to endoplasmic reticulum (ER) stress were measured in FF, and data generated using a de-identified, retrospective, unblinded cohort of human FF and matching demographic and pregnancy outcome data showed that FF levels of ER stress effector proteins GRP78 and CHOP, which are indicative of elevated ER stress in either the oocyte, granulosa cells, or other cells of the follicle, are causally related to poor pregnancy outcomes.
机译:本发明公开了一种用于预测怀孕成功的无偏见的“生物标志物”方法,该方法可在IVF程序中在取卵期间收集的患者卵泡液(FF)中检测到。在FF中测量在细胞对内质网(ER)应激的细胞应答中起作用的蛋白质,并使用未鉴定,回顾性,无盲法的人类FF队列以及相匹配的人口统计学和妊娠结局数据生成的数据显示FF ER应激效应因子水平蛋白GRP78和CHOP指示卵母细胞,颗粒细胞或卵泡其他细胞中的ER应激升高,与妊娠结局不良有因果关系。

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