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Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
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机译:合理设计的具有非回文识别序列的单链大范围核酸酶
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#$%^&*AU2019203619A120190613.pdf#####Abstract: Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites arejoined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.
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