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CONSTRUCTION OF CD27 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF CELL STRAIN HIGHLY EXPRESSING CD27
CONSTRUCTION OF CD27 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF CELL STRAIN HIGHLY EXPRESSING CD27
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机译:CD27真核表达载体的构建及高表达CD27细胞株的制备
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摘要
Provided are construction of a eukaryotic expression vector and a method for preparing a stable-expression cell strain using the vector. Specifically, the method comprises cloning a CD27 full-length gene into a pEGFP-N1 eukaryotic expression vector carrying a fluorescent protein tag, and transfecting Hela cells using the constructed eukaryotic expression vector to establish a HeLa cell line stably overexpressing CD27.
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