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TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST
TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST
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机译:基因组编辑东方伊斯坦布尔 I>和其他工业上有用的酵母的工具和方法
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摘要
The present description relates to genetic tools and methods to facilitate transformation and genetic engineering of industrially-useful yeast/fungal species, such as Issatchenkia orientalis, for which a robust set of genetic tools such as stably inherited and maintained plasmids and functional control sequences is presently lacking. Thus, the present description relates to autonomously replicating sequences (ARSs), RNA polymerase II/III promoters, RNA polymerase II/III terminators, expression cassettes, and vectors comprising same are described herein, as well as uses and methods relating to same, which are functional in I. orientalis.
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机译:本说明书涉及遗传工具和方法,以促进工业上有用的酵母/真菌物种如东方伊氏螯虾的转化和遗传工程,为此,有一套稳健的遗传工具,例如稳定地遗传和维持。目前缺乏质粒和功能控制序列。因此,本说明书涉及本文描述的自主复制序列(ARS),RNA聚合酶II / III启动子,RNA聚合酶II / III终止子,表达盒和包含它们的载体,以及与其相关的用途和方法,在 I. Orientalis I>中起作用。
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