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A new alkaline serine protease named saptex was prepared, purified, physicochemical and molecular characterization. The name of the new alkaline serine protease was saptex. The extract of the new serine protease was extracted from penicillin X5 strain and used to treat protein pollutants on textile carriers.
A new alkaline serine protease named saptex was prepared, purified, physicochemical and molecular characterization. The name of the new alkaline serine protease was saptex. The extract of the new serine protease was extracted from penicillin X5 strain and used to treat protein pollutants on textile carriers.
The present invention relates to uniform purification and biochemical and molecular characterization of an alkaline serine protease ("textile serine alkaline protease") produced by chrysogenium X5 strain. The fungus was isolated from artificially contaminated semi mature lenses. The molecular weight of purified enzyme was estimated to be 10% 43 kDa per SDS PAGE. Sorting of nhzte endThe fruit also proved the purity of the enzyme It showed homogeneity with penicillin serine protease. The optimal protease activity was obtained at pH 10 and 80 ° C. The industrial enzyme was completely inhibited by PMSF and DFP D. the enzyme belongs to serine protease family. The thermal activity and thermal stability were significantly improved by 5 mM calcium. howeverCadmium greatly activated this enzyme activity. The range of pH value (6-12) and high temperature value (80-100 ℃) is very wide. The thermal activity and thermal stability were significantly improved by 5 mM calcium.The serine protease can be used as a biological additive in detergent preparation. It has also been successfully applied to the textile industry. Saptex is also stable in the presence of SDS surfactant.Surfactants (Tween 20, Tween 80 and Triton X-100)2.（Perborate de sodio et h lt sub gt 2 lt；/ In addition, saptex protease was added to detergent solution to improve the performance of desiccant to improve the bleaching of blood pollutants. In addition, saptex protease and commercial protease: saptex, SPTC, flavurzyme 500L, protease, XXII, have a wide range of specificity, and have better catalytic efficiency.Protease K and alkaline 2.4L for casein hydrolysis Therefore, saptex seems to satisfy most of the good proteases used to treat protein contaminants on fabric carriers.
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机译：本发明涉及由产黄杆菌X5菌株产生的碱性丝氨酸蛋白酶（“纺织丝氨酸碱性蛋白酶”）的均匀纯化以及生化和分子表征。从人工污染的半成熟晶状体中分离出真菌。每个SDS PAGE估计纯化的酶的分子量为10％43 kDa。水果末端的分选水果还证明了酶的纯度，与青霉素丝氨酸蛋白酶显示出均一性。在pH 10和80°C时可获得最佳的蛋白酶活性。工业酶被PMSF和DFP D完全抑制。该酶属于丝氨酸蛋白酶家族。 5 mM钙可显着改善热活性和热稳定性。然而，镉极大地激活了这种酶的活性。 pH值（6-12）和高温值（80-100℃）的范围非常宽。 5 mM钙显着提高了热活性和热稳定性。丝氨酸蛋白酶可作为洗涤剂制备中的生物添加剂。它也已成功地应用于纺织工业。 Saptex在SDS表面活性剂的存在下也是稳定的。表面活性剂（Tween 20，Tween 80和Triton X-100）2。（Perborate de sodio et h lt gt 2 /另外，将saptex蛋白酶添加到洗涤剂溶液中以改善干燥剂的性能，改善血液污染物的漂白。此外，saptex蛋白酶和商业蛋白酶：saptex，SPTC，黄素酶> 500L，蛋白酶，XXII具有广泛的特异性，并且具有更好的催化效率。酪蛋白水解的K蛋白酶和碱性2.4L蛋白酶因此，saptex似乎最满足好的蛋白酶用于处理织物载体上的蛋白质污染物。
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