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ALGAL MUTANTS HAVING A LOCKED-IN HIGH LIGHT ACCLIMATED PHENOTYPE

机译:藻类突变体具有锁定的高光适应性表型

摘要

Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher P max per unit chlorophyll with little to no reduction in P max per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.
机译:提供了具有减少的叶绿素和增加的光合作用效率的突变的光合微生物。突变体具有高光适应性表型,在低光照条件下,LIHLA突变体发现了高光适应性野生型细胞的许多光合参数,例如叶绿素减少,NPQ降低,qP较高, Ek较高,每单位叶绿素的Pmax较高,而每个细胞的Pmax几乎没有或没有降低,并且在较大的光强度范围内,通过光系统II的电子传输速率较高。本文提供了用于减弱或破坏基因的构建体,提供了用于产生具有LIHLA表型的突变体的基因。还提供了培养LIHLA突变体以生产生物质或其他产物的方法。

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