首页> 外国专利> A method for confirming the detection and enumeration of suspected target cells in a blood sample comprising a mixed cell population.

A method for confirming the detection and enumeration of suspected target cells in a blood sample comprising a mixed cell population.

机译:一种用于确认包括混合细胞群的血样中​​可疑靶细胞的检测和计数的方法。

摘要

a blood sample containing ctcs, or other cells of interest, is labeled with fluorescent markers for image analysis and screened for presence and position within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets can be retested with additional biomarkers conjugated to the same or different fluorochromes using the same imaging criteria that were used. for the initial analysis. The present invention has applications with targets such as circulating epithelial cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their residues, or any other formed element that could be captured and generate image. The invention provides an additional means of interrogating individual targets of interest, especially when paired with genetic analysis, such as fish.
机译:用荧光标记物标记含有ctcs或其他感兴趣细胞的血液样品,以进行图像分析,并筛选靶细胞或亚细胞元件盒中的存在和位置。然后进一步处理包含所需靶细胞或亚细胞成分的样品,部分方法是通过光漂白该样品,以便可以使用与所使用的相同成像标准结合到相同或不同荧光染料上的其他生物标记物,对那些相同的靶标进行重新测试。进行初步分析。本发明具有针对靶标的应用,例如循环上皮细胞,循环肿瘤细胞,循环内皮细胞,白细胞,淋巴细胞亚群,含有目的细胞器或受体的细胞,细胞碎片,破裂的细胞及其残基或任何其他形成的元素可以被捕获并生成图像。本发明提供了询问特别是与诸如鱼之类的遗传分析配对的目标个体的附加手段。

著录项

  • 公开/公告号BR122018015761B1

    专利类型

  • 公开/公告日2019-10-22

    原文格式PDF

  • 申请/专利权人 VERIDEX LLC;

    申请/专利号BR20181215761

  • 申请日2009-07-27

  • 分类号G01N33/49;G01N33/52;G01N33/53;G01N33/574;

  • 国家 BR

  • 入库时间 2022-08-21 12:03:25

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