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LINKED PERFUSION TO CONTINUOUS-FLOW STIRRED-TANK REACTOR CELL CULTURE SYSTEM

机译:连续灌注搅拌罐反应器细胞培养系统的链接灌注

摘要

Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.
机译:提供了在链接的培养和生产生物反应器系统中生产蛋白质的方法。这样的方法包括与生产生物反应器(N生物反应器)连接的培养生物反应器(N-1生物反应器)。更具体地说,这些方法包括:(a)在连续灌注培养生物反应器(N-1生物反应器)中用编码目的蛋白的基因培养细胞;用从步骤(a)获得的细胞接种连续搅拌釜反应器(CSTR)生产生物反应器(N生物反应器);在允许产生目的蛋白质的条件下,在CSTR生产生物反应器中培养细胞。

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