首页> 外国专利> MULTI-TIERED HIGH THROUGH-PUT SCREEN FOR COMPOUNDS EFFECTIVE AGAINST BACTERIAL BIOFILM AND COMPOUNDS EFFECTIVE FOR INHIBITING AND ERADICATING BACTERIAL BIOFILM

MULTI-TIERED HIGH THROUGH-PUT SCREEN FOR COMPOUNDS EFFECTIVE AGAINST BACTERIAL BIOFILM AND COMPOUNDS EFFECTIVE FOR INHIBITING AND ERADICATING BACTERIAL BIOFILM

机译：抗菌生物膜有效成分的多层高通量筛选及抗菌生物膜的根除有效化合物

页面导航

摘要
著录项
相似文献

摘要

A high through-put screening method for identifying agents effective for inhibiting biofilm formation and/or killing established biofilm are disclosed. The method includes three tiers, and each tier includes three specific biological process assays. The tier levels are a primary screen, a confirmation screen, and a dose-response screen, and the biological process assays include assays for total bacterial growth, bacterial metabolic activity, and biofilm formation. The series of assays may be run once or more than once at each tier. A library of compounds is subject to tier A and only compounds meeting a primary parameter advance to tier B, and only tier B compounds meeting a confirmation parameter advance to tier C, and only tier C compounds meeting a dose-response parameter are identified as putative agents effective for inhibiting and/or eradicating a biofilm, further wherein the assays are conducted for each compound subject to the respective tier. The method is effective and validated for identifying agents which inhibit and/or kill Staphylococcus epidermidis, Pseudomonas aeruginosa, and Acinetobacter baumannii biofilms. Agents identified according to the high through-put screen and validated in follow-up experiments as effective for inhibiting and/or killing bacterial biofilms are also disclosed.

机译：公开了一种高通量筛选方法，用于鉴定对抑制生物膜形成和/或杀死已建立的生物膜有效的试剂。该方法包括三个层次，每个层次包括三个特定的生物学过程分析。层级是主要筛选，确认筛选和剂量响应筛选，生物过程分析包括总细菌生长，细菌代谢活性和生物膜形成的分析。一系列测定可以在每个层运行一次或多次。化合物库属于A级化合物，只有满足主要参数的化合物才达到B级，只有满足确认参数的B类化合物才达到C级，并且只有满足剂量反应参数的C类化合物才被认为是推定的对于抑制和/或消除生物膜有效的药物，进一步地，其中对每种化合物分别进行相应的测定。该方法是有效的，可用于鉴定抑制和/或杀死表皮葡萄球菌，铜绿假单胞菌和鲍曼不动杆菌生物膜的药剂。还公开了根据高通量筛选鉴定并在后续实验中验证为有效抑制和/或杀死细菌生物膜的药剂。

著录项

公开/公告号US2019127776A1

专利类型
公开/公告日2019-05-02

原文格式PDF
申请/专利权人 UNIVERSITY OF CINCINNATI;GOVERNMENT OF THE UNITED STATES AS REPRESENTED BY THE SECRETARY OF THE AIR FORCE;
展开▼

申请/专利号US201816224006
发明设计人 DANIEL J. HASSETT;THOMAS J. LAMKIN;WARUNYA PANMANEE;DEBORAH E. TAYLOR;CHLOE J.A. SHEA;
展开▼

申请日2018-12-18
分类号C12Q1/18;C07D405/14;A61K31/175;C07D471/04;C07C243/28;C07C311/21;C07D209/08;C07D215/52;C07D215/56;C07D231/20;C07D235/18;C07D271/12;C07D275/03;C07D307/92;C07D401/04;C07D405/04;C07D413/04;A01N37/10;A01N37/28;A01N41/06;A01N43/12;A01N43/38;A01N43/42;A01N43/44;A01N43/52;A01N43/54;A01N43/56;A01N43/80;A01N43/82;A01N43/90;A01N47/38;C07C69/738;A61K31/519;A61K31/506;A61K31/496;A61K31/4709;A61K31/4545;A61K31/435;A61K31/425;A61K31/4245;A61K31/4184;A61K31/4155;A61K31/404;A61K31/343;A61K31/216;A61K31/18;C07D491/052;
国家 US
入库时间 2022-08-21 12:06:25

相似文献

专利
外文文献
中文文献