Protein Chip, Kit and Preparation Method thereof for Detecting Abnormal Decarboxy Prothrombin in Serum

• 1. 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 [P] . 中国专利： CN107727864A . 2018-02-23
• 2. 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 [P] . 中国专利： CN107748261A . 2018-03-02
• 3. A Protein Chip and Kit for Detecting Abnormal Des-carboxy-prothrombin (DCP) in Serum and the Preparing Methods Thereof [P] . AU2016101575A4 . 2016-10-06

  机译： 一种检测血清中des-羧基-凝血酶原异常的蛋白芯片及试剂盒及其制备方法

• 4. Protein chip, kit, and manufacturing method for detecting abnormal decarboxylase prothrombin in serum [P] . KR102266095B1 . 2021-06-18

  机译： 蛋白质芯片，试剂盒和用于检测血清中异常脱羧酶凝血酶酶的制造方法

• 5. Protein chip, kit and method for detecting abnormal dicarboxylase prothrombin in serum [P] . 韩国专利： KR20190026825A . 2019-03-13

  机译： 检测血清中二羧化酶凝血酶原异常的蛋白质芯片，试剂盒和方法

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Using the SELDI Protein Chip System to Detect Changes in Protein Expression in Vero Cells after Infection [J] . Zhi-jun LIU ,Bin WANG ,Zhi-yong YAN . 中国病毒学 . 2007,第001期
• 3. 甲胎蛋白(AFP)、甲胎蛋白异质体比例(AFP-L3％)及血清异常凝血酶原(DCP)在原发性肝癌中的诊断价值 [C] . 顾志冬 ,陈诗凯 ,郭平 . 第五次全国免疫诊断暨疫苗学术研讨会 . 2011
• 4. 血清异常凝血酶原-Ⅱ在原发性肝癌中的应用研究 [A] . 李俊利 . 2018