METHOD AND KIT FOR DETECTING BACTERIUM OF GENUS NOVOSPHINGOBIUM

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• 2. 一种同时检测弧菌属细菌和鮰爱德华氏菌的试剂盒和检测方法 [P] . 中国专利： CN105132411A . 2015-12-09
• 3. Method and kit for detecting bacterium of genus Novosphingobium [P] . US11203789B2 . 2021-12-21

  机译： 检测Novosphillobium属细菌的方法和试剂盒

• 4. METHOD AND KIT FOR DETECTING BACTERIUM OF GENUS NOVOSPHINGOBIUM [P] . 世界知识产权组织专利： WO2018003804A1 . 2018-01-04

  机译： 检测异型鞘氨醇细菌的方法和试剂盒

• 5. OLIGONUCLEOTIDE FOR DETECTING BACTERIUM BELONGING TO GENUS CAMPYLOBACTER, METHOD FOR DETECTING BACTERIUM BELONGING TO GENUS CAMPYLOBACTER AND REAGENT KIT FOR DETECTION [P] . 日本专利： JPH05276997A . 1993-10-26

  机译： 用于检测属于细菌的细菌的寡核苷酸，用于检测细菌的细菌的方法和用于检测的试剂盒

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods,using four commercially kits [J] . Zsolt Kovacs ,Ioan Jung ,Erzsebet Csernak . 世界临床肿瘤学杂志：英文版 . 2019,第9期
• 3. 大肠杆菌属细菌的LAMP检测方法 [C] . 林超 ,贾俊涛 ,刘云国 . 2011食品安全技术与标准国际研讨会暨AOAC中国区年会 . 2011
• 4. DNA条形码试剂盒在疫霉属和腥黑粉菌属检测中的应用及风险分析 [A] . 李晓琪 . 2015