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COMPOSITIONS AND METHODS OF MODIFYING A PLANT GENOME TO PRODUCE A MS9, MS22, MS26, OR MS45 MALE-STERILE PLANT

机译:修饰植物基因组以生产MS9,MS22,MS26或MS45男性不育植物的组合物和方法

摘要

Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.
机译:提供了用于对植物细胞或植物基因组中MS9,MS22,MS26或MS45的雄性育性基因中或附近的核苷酸序列进行基因组修饰的组合物和方法,以产生雄性不育植物。在一些实例中,所述方法和组合物采用指导RNA / Cas核酸内切酶系统来修饰或改变位于植物细胞,植物或种子的基因组中的MS9,MS22,MS26或MS45的雄性育性基因中或附近的靶位点。生产雄性不育植物。还提供了使用指导性多核苷酸/ Cas核酸内切酶系统进行基因组修饰的组合物和方法,所述核苷酸序列位于植物细胞的基因组中MS9,MS22,MS26或MS45的雄性育性基因中或附近的核苷酸序列以产生雄性不育。厂。还提供了用于使使用本文所述的方法和组合物产生的雄性不育的Ms9,Ms22,Ms26或Ms45植物的Ms9,Ms22,Ms26或Ms45核苷酸序列的育性恢复的组合物和方法。

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