The purpose of the present invention is to provide: a non-specific adsorption inhibitor which is applicable as a blocking agent for western blotting methods that utilize various antigen-antibody reactions, and which enables an analysis at a high S/N ratio with high sensitivity and high accuracy; a reagent for western blotting methods, which contains this non-specific adsorption inhibitor; and a blocking method, in which blocking is carried out with use of this non-specific adsorption inhibitor.The preset invention relates to: a non-specific adsorption inhibitor for western blotting methods, which contains a copolymer that has a repeating unit represented by general formula [1] and a repeating unit represented by general formula [2]; a reagent for western blotting methods, which contains this non-specific adsorption inhibitor; and a blocking method, in which blocking is carried out with use of this non-specific adsorption inhibitor.(In general formulae [1] and [2], each of X1 and X2 independently represents an oxygen atom or an -NH- group; each of R1 and R2 independently represents a hydrogen atom or an alkyl group having 1-6 carbon atoms; each of R3 and R5 independently represents a hydrogen atom or an alkyl group having 1-3 carbon atoms; R4 represents an alkyl group having 1-10 carbon atoms, an aryl group having 6-10 carbon atoms or an arylalkyl group having 7-12 carbon atoms; m represents an integer of 1-6; and n represents an integer of 1-3.)
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机译：本发明的目的是提供：一种非特异性吸附抑制剂，其可用作利用各种抗原-抗体反应的蛋白质印迹方法的封闭剂，并能够以高信噪比进行高灵敏度分析。精度高;用于蛋白质印迹法的试剂，其中包含这种非特异性吸附抑制剂;封闭方法，其中使用该非特异性吸附抑制剂进行封闭。本发明涉及：一种用于蛋白质印迹法的非特异性吸附抑制剂，其包含具有通式[1]表示的重复单元和通式[2]表示的重复单元的共聚物。用于蛋白质印迹法的试剂，其中包含这种非特异性吸附抑制剂;封闭方法，其中使用该非特异性吸附抑制剂进行封闭。（在通式[1]和[2]中，X 1 和X 2 各自独立地表示一个氧原子或-NH-基团; R 1 和R 2 独立代表氢原子或碳原子数为1-6的烷基; R 3 和R 5 < / Sup>独立地代表氢原子或具有1-3个碳原子的烷基; R 4 代表具有1-10个碳原子的烷基，具有6-10个碳原子的芳基或具有7-12个碳原子的芳烷基; m表示1-6的整数; n表示1-3的整数。）
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