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Method for producing high-quality medium composition for three-dimensional culture, and method for evaluating storage stability of medium composition for three-dimensional culture

机译:一种用于三维培养的高质量培养基组合物的生产方法以及一种用于三维培养的培养基组合物的贮存稳定性的评价方法

摘要

This invention is a manufacturing method of a liquid culture medium composition, Comprising: The 1st liquid containing the specific compound of the following (i), and the 2nd liquid containing the connection substance of the following (ii), 25 Mixing at a temperature not higher than ° C. and exceeding the freezing point, and forming a liquid medium composition containing the structure in which the specific compound is bonded through the connecting substance in a uniformly dispersed state. Provided is a method for producing a medium composition: (i) a polymer having an anionic functional group capable of forming a structure capable of floating a cell or tissue by binding via a divalent metal cation A specific compound which is a compound, (ii) a linking substance which is a divalent metal cation.
机译:本发明是液体培养基组合物的制造方法,其特征在于,在以下温度下混合:第1液体含有下述(i)的特定化合物,第2液体含有下述(ii)的连接物质。高于℃并超过冰点,并形成液体介质组合物,其包含其中特定化合物通过连接物质以均匀分散状态结合的结构。提供了一种制备培养基组合物的方法:(i)具有阴离子官能团的聚合物,该阴离子官能团能够通过经由二价金属阳离子结合而形成能够使细胞或组织漂浮的结构。作为化合物的特定化合物,(ii)作为二价金属阳离子的连接物质。

著录项

  • 公开/公告号JPWO2018079797A1

    专利类型

  • 公开/公告日2019-09-19

    原文格式PDF

  • 申请/专利权人 日産化学株式会社;

    申请/专利号JP20180547842

  • 发明设计人 金木 達朗;猿橋 康一郎;

    申请日2017-10-31

  • 分类号C12N1;

  • 国家 JP

  • 入库时间 2022-08-21 12:18:28

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