首页> 外国专利> METHOD FOR DETERMINING THE IN VITRO PRESENCE OF A TARGET MOLECULE IN A BIOLOGICAL SAMPLE, AND ITS USE FOR THE IN VITRO ANALYSIS OF CANCERS, IN PARTICULAR SOFT-TISSUE SARCOMAS AND GASTROINTESTINAL STROMAL TUMORS

METHOD FOR DETERMINING THE IN VITRO PRESENCE OF A TARGET MOLECULE IN A BIOLOGICAL SAMPLE, AND ITS USE FOR THE IN VITRO ANALYSIS OF CANCERS, IN PARTICULAR SOFT-TISSUE SARCOMAS AND GASTROINTESTINAL STROMAL TUMORS

机译：确定生物样品中目标分子的体外存在的方法及其在癌症，特殊软组织肉瘤和胃肠道间质瘤中的体外分析中的用途

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摘要

The present invention relates to a method for determining in vitro the presence and/or the level of target molecules in a biological sample, comprising the steps of:(1) placing said biological sample in the presence of a pool of different probes, each probe comprising (a) a binding region specific of one target molecule, and eventually (b) a detectable region that is unique for each of the at least one target molecule, wherein the binding region of a probe comprises a polynucleotide having a sequence selected in the group consisting of SEQ ID NO: 1 to SEQ ID NO: 67 or of SEQ ID NO: 1 to SEQ ID NO: 75, and wherein the presence of a target molecule in the biological sample allows the formation of a specific detectable molecular complexes between said probe and said target molecule, and(2) detecting and/or counting the molecular complex(es) formed, thus allowing the determination of the presence and/or of the level of said at least one target molecule in the biological sample.;The present invention also relates to the use of the method for the in vitro analysis of a cancer.;The present invention also relates to a pool of polynucleotides comprising the polynucleotides of sequence SEQ ID NO : 1 to SEQ ID NO : 67 or SEQ ID NO : 1 to SEQ ID NO : 75, and to its use in a method for determining in vitro the presence and/or the level of at least one target molecule in a biological sample.

机译：本发明涉及一种用于在体外确定生物样品中靶分子的存在和/或水平的方法，该方法包括以下步骤：（1）将所述生物样品置于不同探针池的存在下，每个探针包含（a）对一个靶分子具有特异性的结合区，以及最终（b）对于至少一个中的每一个而言唯一的可检测区。靶分子，其中探针的结合区包含具有选自SEQ ID NO：1至SEQ ID NO：67或SEQ ID NO：1至SEQ ID NO：75的序列的多核苷酸，并且其中生物样品中靶分子的存在允许在所述探针和所述靶分子之间形成特异性可检测的分子复合物，并且（2）检测和/或计数形成的分子复合物，从而允许确定生物样品中所述至少一种靶分子的存在和/或水平。;本发明还涉及用途本发明还涉及多核苷酸库，其包含序列SEQ ID NO：1至SEQ ID NO：67或SEQ ID NO的多核苷酸：SEQ ID NO：75至SEQ ID NO：75，以及其在用于确定体外的方法中的用途，该方法在生物样品中至少一种靶分子的存在和/或水平。 展开▼

著录项

公开/公告号EP3530758A1

专利类型

公开/公告日2019-08-28

原文格式PDF

申请/专利权人 UNIVERSITÉ DE BORDEAUX;INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE);INSTITUT BERGONIÉ;UNIVERSITÉ PAUL SABATIER TOULOUSE III;INSTITUT CLAUDIUS REGAUD;
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申请/专利号EP18305190.3

发明设计人 CHIBON FRÉDÉRIC;LESLUYES TOM;LE GUELLEC SOPHIE;
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申请日2018-02-23

分类号C12Q1/6886;

国家 EP

入库时间 2022-08-21 12:27:22

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机译：确定生物样品中目标分子的体外的方法，并用于癌症，特殊软组织肉瘤和胃肠道基质中的体外分析肿瘤

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9. 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