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IN VITRO METHOD FOR IDENTIFICATION AND ANALYSIS OF PROTEINS WITH STEM CELL FUNCTION USING A THREE-DIMENSIONAL CELL CULTURE MODEL OF THE SWEAT GLAND
IN VITRO METHOD FOR IDENTIFICATION AND ANALYSIS OF PROTEINS WITH STEM CELL FUNCTION USING A THREE-DIMENSIONAL CELL CULTURE MODEL OF THE SWEAT GLAND
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机译:利用三维腺细胞培养模型体外鉴定和分析具有干细胞功能的蛋白质
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摘要
The present disclosure concerns an in-vitro method for the identification and analysis of proteins with a stem cell function, in which initially, at least one three-dimensional sweat gland equivalent with from about 500 to about 500000 sweat gland cells as well as a diameter of from about 100 to about 6000 μm is provided and subsequently, proteins with a stem cell function in this equivalent are identified and analyzed. Preferably, in a further step c) of the method, the influence of test substances on the proteins previously identified in step b) is investigated. Because the three-dimensional sweat gland equivalents used in step a) comprise differently differentiated cells and emulate the in-vivo situation well, the measured data obtained with the in-vitro method as contemplated herein can readily be applied to the in-vivo situation.
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