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Device and method for spatially resolved fluorescence correlation spectroscopy
Device and method for spatially resolved fluorescence correlation spectroscopy
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机译：空间分辨荧光相关光谱的装置和方法
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摘要
Method for spatially resolved fluorescence correlation spectroscopy (FCS) with a sample (P), with (a) an imaging laser scanning microscope unit, LSM unit, with at least one LSM detector, (b) a fluorescence correlation spectroscopy unit, FCS unit, for analyzing molecular interactions in small volumes by means of fluorescence correlation spectroscopy, characterized in that (c) measuring sites for the analysis of molecular interactions are determined and selected using the imaging method at least two-dimensionally, (d) both the LSM imaging unit and the FCS unit are operated with a common control unit (CU), (e) the analysis results are assigned to the image of the LSM imaging unit and (F) via the control unit (CU) and a computer (C) at least the analysis results of the FCS unit are displayed pictorially, (g) whereby the sample (P) is scanned point by point at least two-dimensionally with illumination light, (H) the light coming from the sample (P) is detected via at least one first detector and (i) during the scanning process and / or after the scan for at least one sample point an FCS evaluation takes place whereby the FCS unit between the scanner (S) of the LSM unit and the sample (P) coupled into the illumination beam path of the LSM unit is.
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