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首页> 外国专利> METHOD FOR DETERMINATION OF PREPARATION INFLUENCE ON INTERACTION OF COMPLEMENT WITH ANTIGEN-ANTIBODY COMPLEX

METHOD FOR DETERMINATION OF PREPARATION INFLUENCE ON INTERACTION OF COMPLEMENT WITH ANTIGEN-ANTIBODY COMPLEX

机译：测定制剂对抗原与抗原复合物相互作用的影响的方法

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FIELD: chemistry.;SUBSTANCE: invention relates to medicine, namely biological chemistry, and can be used to determine the effect of drugs on the classical pathway of complement activation by studying the hemolytic activity of complement. To do this, 50 mcl of blood serum from ½ to 1/128 of blood serum are added to 50 mcl each before dilution in a saline solution to a concentration of 10-2 M, preliminary incubation is carried out at a temperature of 4–6 °C for 30 minutes, then add 50 mcl of sensitized erythrocytes of ram and veronal buffer. Then the cells are re-incubated, the supernatant is taken out and its optical density is determined at 450 nm, and complement fixation (C%) with sensitized erythrocytes in the presence of the studied preparations is calculated by the formula: C%=(Eo-Ec)/Eo-100, where: Eo – optical density in the presence of the preparation, Ec – the optical density of the control, which is used as a veronal buffer. Value of C is judged on the effect of the drug on the C1q subcomponent of the first component of the classical complement pathway in terms of the degree of hemolysis of the sensitized erythrocytes of the sheep.;EFFECT: use of this method makes it possible to make a comparative quantitative assessment of the degree of influence of the tested drugs on the classical pathway of complement activation.;1 cl, 2 dwg, 2 ex
机译：技术领域本发明涉及医学，即生物化学，并且可以通过研究补体的溶血活性来确定药物对补体激活经典途径的作用。为此，在盐溶液中稀释至浓度为10 -2 之前，将50 mcl的血清从½到1/128的血清分别添加到50 mcl，进行初步孵育在4–6°C的温度下放置30分钟，然后添加50 mcl的ram和veronal缓冲液致敏的红细胞。然后将细胞重新温育，取出上清液，并在450 nm下测定其光密度，并在存在所研究制剂的情况下用致敏红细胞补体固定（C％）由下式计算：C％=（ E o -E c ）/ E o -100，其中：E o –存在时的光密度制剂中的E c –对照的光密度，用作Veronal缓冲液。根据绵羊对致敏性红细胞的溶血程度，可根据药物对经典补体途径第一个成分的C1q子成分的作用来判断C的值。效果：使用此方法可以对被测药物对补体激活经典途径的影响程度进行比较定量评估。; 1 cl，2 dwg，2 ex

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