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RECOMBINANT PLASMID DNA pPDGFB ENCODING A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES, AND A RECOMBINANT STRAIN OF METHYLOTROPHIC YEAST PICHIA PASTORIS, A PRODUCER OF A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES
RECOMBINANT PLASMID DNA pPDGFB ENCODING A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES, AND A RECOMBINANT STRAIN OF METHYLOTROPHIC YEAST PICHIA PASTORIS, A PRODUCER OF A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES
FIELD: biotechnology.;SUBSTANCE: present invention relates to biotechnology and in particular to genetic engineering and can be used in medical studies to obtain a human platelet growth factor. Recombinant plasmid DNA pPDGFB encoding the synthesis of a polypeptide with human platelet-BB growth factor, which provides biosynthesis with a level of about 40÷60 mg of recombinant protein/liter culture. Highly efficient synthesis of the target polypeptide is ensured by the fact that the target gene is under the control of the strong inducible promoter AOX1.;EFFECT: present Pichia pastoris strain GS115PDGFB provides the synthesis of a polypeptide with human platelet-BB growth factor in an amount of at least 40 mg of the target protein per liter of culture.;2 cl, 4 dwg, 3 ex
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机译：技术领域本发明涉及生物技术，尤其涉及基因工程，并且可以在医学研究中用于获得人血小板生长因子。重组质粒DNA pPDGFB编码具有人血小板BB生长因子的多肽的合成，可提供约40÷60 mg重组蛋白/升培养物水平的生物合成。靶基因在强诱导型启动子AOX1的控制下，可确保靶多肽的高效合成。效果：目前的巴斯德毕赤酵母菌株GS115PDGFB可以合成具有人血小板BB生长因子的多肽。每升培养物中至少40 mg的目标蛋白量; 2 cl，4 dwg，3 ex
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