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首页> 外国专利> METHOD FOR OBTAINING ORGAN CULTURE OF A TRABECULAR NETWORK FROM A HUMAN CADAVER EYE

METHOD FOR OBTAINING ORGAN CULTURE OF A TRABECULAR NETWORK FROM A HUMAN CADAVER EYE

机译：从人鹰眼获得管网的器官文化的方法

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FIELD: medicine.;SUBSTANCE: invention relates to medicine, namely to ophthalmology and transplantology. To obtain the organ culture of the trabecular network from the human cadaveric eye, a circular incision of the sclera and the vascular membrane is performed, separation of the anterior pole of the eye (PSG), removal of the lens, and the separation of the PSG into parts. Donor cadaverous eye is fixed in the eyeball holder until a tonus of the eye of a living person is reached. Produce a scarification of the epithelium from the surface of the cornea and completely excise the conjunctival tissue. Before the circular incision of the sclera and the choroid, a scleral incision is made at a distance of 2 mm from the limbus with a depth not exceeding 0.1 mm. Circular trephine with a diameter of 8 mm excised and removed the remaining layers of the cornea, excluding its transition to the sclera. Then scissors make excision of the sclera and underlying tissues according to the previously outlined incision, resulting in a sample of the trabecular network, including trabeculae tissue, iris and the transitional part of the cornea in the sclera. Place the sample on the bottom of the sterile petri dish with the corneal side down and dissect it meridionally into 4 parts. Further, by magnification of the 20X microscope, iris excision is performed in each part, leaving 2 mm of tissue at its root. Then the obtained parts of the trabecular network are placed in a solution of the culture medium of the following composition: per 10 ml of medium 8,900 mcl of DMEM/F12, 1,000 mcl of 10 % fetal bovine serum, 100 mcl of 2 mM L-glutamine, 100 mcl of an antibiotic-antimycotic solution, including 10,000 IU/ml penicillin, 10,000 mcg/ml of streptomycin, 25 mcg/ml amphotericin and cultivated at 37 °C, 5 % CO2 content and humidity 100 %. Medium is changed every 3 days.;EFFECT: method allows to increase the reliability of the results in modeling the in vivo state for the purpose of studying in the experiment, by obtaining intact, viable trabecular tissue with preservation of intravital functions and with the least amount of surrounding tissues.;1 cl, 1 ex, 1 dwg
机译：技术领域本发明涉及医学，即涉及眼科和移植学。为了从人尸眼获得小梁网络的器官培养物，进行巩膜和血管膜的圆形切口，分离眼的前极（PSG），摘除晶状体，并分离晶状体。 PSG分为多个部分。供体尸体的眼睛固定在眼球支架中，直到达到活人的眼球。从角膜表面产生上皮的划痕，并完全切除结膜组织。在巩膜和脉络膜的圆形切口之前，在距角膜缘2 mm处切开巩膜切口，深度不超过0.1 mm。切下直径为8 mm的圆形环苯丙氨酸，除去角膜向巩膜的过渡部分，除去角膜的其余层。然后用剪刀根据先前概述的切口切除巩膜和下面的组织，得到小梁网络的样本，包括小梁组织，虹膜和巩膜中角膜的过渡部分。将样品放在无菌陪替氏培养皿的底部，使角膜的一面朝下，然后将其子午地分成4部分。此外，通过放大20倍显微镜，虹膜切除术在每个部分进行，在其根部留下2 mm的组织。然后将获得的小梁网络部分放入具有以下成分的培养基溶液中：每10 ml培养基8,900 mcl DMEM / F12、1,000 mcl 10％胎牛血清，100 mcl 2 mM L-谷氨酰胺，100毫升的抗生素-抗真菌溶液，包括10,000 IU / ml的青霉素，10,000 mcg / ml的链霉素，25 mcg / ml的两性霉素并在37°C下培养，CO 2 含量为5％湿度100％每3天更换一次培养基;效果：该方法可通过获取完整，可行的小梁组织并保留玻璃体内功能，并且最少地获得结果，从而提高在体内状态建模以供实验研究的结果的可靠性周围组织的数量。; 1 cl，1 ex，1 dwg

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