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METHOD FOR RESEARCHING INCREASE OF ANTIBIOTIC SENSITIVITY OF CONDITIONALLY-PATHOGENIC MICROFLORA IN VITRO WITH MILK-FOUNDATION FODDER ADDITIVE CONTAINING STREPTOCOCCUS TERMOPHILUS TH-4 MICROORGANISM CULTURE
METHOD FOR RESEARCHING INCREASE OF ANTIBIOTIC SENSITIVITY OF CONDITIONALLY-PATHOGENIC MICROFLORA IN VITRO WITH MILK-FOUNDATION FODDER ADDITIVE CONTAINING STREPTOCOCCUS TERMOPHILUS TH-4 MICROORGANISM CULTURE
FIELD: microbiology.SUBSTANCE: invention relates to veterinary microbiology and can be used in laboratory practice in the study of the antibiotic susceptibility of microorganisms. Method of research increase in antibiotic sensitivity of conditionally pathogenic microflora in vitro with a lactic feed additive containing a Streptococcus termophilus TH-4 microorganism culture, includes the use of the supernatant of a lactic acid feed additive containing Streptococcus termophilus TH-4 microorganism culture, obtained in different periods of cultivation – at 1, 10, 20 and 30 days, then adding 0.5 ml of the supernatant of the lactic acid feed additive to 0.5 ml of the BCH medium, adding 0.5 ml of the BCH of the daily culture of one of the strains E. fecalis 200, St. albus ATCC 25923, E. coli ATCC 25222, Pr. vulgaris 192, Kl. pneumonia 71 of conditionally pathogenic microflora, the resulting solution is incubated for 18–24 h at T=37.0±0.5 °C, then carrying out the determination of the antibiotic sensitivity of microorganisms by disc diffusion method on MPA with 24 types of antibacterial drugs.EFFECT: method allows to expand the arsenal of methods for increasing the antibiotic sensitivity of microorganisms in vitro.1 cl, 2 tbl, 2 ex
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