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A Method for promoting proliferation and osteogenic differentiation of Human Alveolar Bone-derived Mesenchymal Stem Cells and a composition therefor

机译:一种促进人牙槽骨间充质干细胞增殖和成骨分化的方法及其组合物

摘要

The present invention relates to a method for culturing alveolar bone-derived mesenchymal stem cells (ABMSCs) in α-minimal essential medium containing β-glycerophosphate and ascorbic acid, The cells were cultured under fluid shear stress during the incubation period, and after 4-6 days, the culture medium was collected to remove cell debris, filtered using a filter, and then cultured in a medium containing the harvested medium as an active ingredient The present invention provides a composition for alveolar bone-derived mesenchymal stem cell proliferation and osteogenic differentiation promotion. The composition of the present invention promotes alveolar bone-derived mesenchymal stem cell proliferation and osteogenesis differentiation, synergistically promotes differentiation and maturation of alveolar bone-derived mesenchymal stem cells, and induces osteoblast differentiation.
机译:本发明涉及一种在含有β-甘油磷酸和抗坏血酸的α-最小必需培养基中培养牙槽骨来源的间充质干细胞(ABMSC)的方法,在培养过程中以及在培养后4- 6天,收集培养基以去除细胞碎片,使用过滤器过滤,然后在包含收获的培养基作为活性成分的培养基中培养。本发明提供了用于牙槽骨来源的间充质干细胞增殖和成骨分化的组合物。晋升。本发明的组合物促进牙槽骨来源的间充质干细胞的增殖和成骨分化,协同地促进牙槽骨来源的间质干细胞的分化和成熟,并诱导成骨细胞分化。

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