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How to determine the origin tidal utilizing the complex stable isotopic analysis of bivalves

机译:如何利用双壳类动物的复杂稳定同位素分析确定潮汐起源

摘要

The present invention provides a method for distinguishing each origin place of a domestic bivalve and an imported bivalve through a stable isotope ratio analyzing method using content of carbon and sulfur, which is a specific element having an isotope contained in a bivalve in a mud flat and an isotope content ratio of each element derived from the content of carbon and sulfur. The method for distinguishing an origin place of a bivalve in a mud flat by using a stable isotope ratio analyzing method comprises: (a) a step of pulverizing a sample after selecting a kind of a bivalve for distinguishing an origin place and freezing and drying the bivalve, removing lipid from the pulverized sample, and then, removing inorganic carbons to analyze a stable isotope ratio; (b) a linear discriminant analysis calculation step of analyzing multi-dimensional data with a stable isotope ratio-measured value completely analyzed in step (a) and providing a statistical probability value; and (c) a step of providing information on determination of classification of an origin place in accordance with step (b). According to this method, reliability further improved rather than a conventional carbon and nitrogen stable isotope ratio is shown, thereby preventing an error in distinguishing a place of origin, which may occur when genetic information is equal even when the habitat is different.
机译:本发明提供一种通过使用碳和硫的含量通过稳定的同位素比分析方法来区分家用双壳类动物和进口双壳类动物的每个产地的方法,碳和硫是在泥滩中双壳类动物体内具有同位素的特定元素。由碳和硫的含量得出的每种元素的同位素含量比。通过使用稳定的同位素比分析方法来区分泥滩中双壳类动物的起源地的方法包括:(a)在选择用于区分起源地的双壳类动物的一种之后,将样品粉碎并冷冻干燥的步骤。双瓣,从粉碎的样品中除去脂质,然后除去无机碳以分析稳定的同位素比; (b)线性判别分析计算步骤,以步骤(a)中完全分析的稳定同位素比测量值分析多维数据,并提供统计概率值; (c)根据步骤(b)提供有关确定原产地的分类的信息的步骤。根据该方法,与传统的碳和氮稳定同位素比相比,显示了可靠性的进一步提高,从而防止了在区分起源地时的错误,即使在生境不同的情况下,当遗传信息相等时,也可能发生这种错误。

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