METHODS AND COMPOSITION FOR DETECTING EARLY STAGE COLON CANCER WITH RNA-SEQ EXPRESSION PROFILING

• 1. 一种特征lincRNA表达谱组合及结肠癌的早期预测方法 [P] . 中国专利： CN111748634A . 2020-10-09
• 2. 一种特征mRNA表达谱组合及结肠癌早期预测方法 [P] . 中国专利： CN111951883A . 2020-11-17
• 3. METHODS AND COMPOSITIONS FOR DETECTING EARLY STAGE BLADDER CANCER WITH RNA-SEQ EXPRESSION PROFILING [P] . 世界知识产权组织专利： WO2018174862A1 . 2018-09-27

  机译： RNA序列表达谱分析检测早期膀胱癌的方法和组成

• 4. METHODS AND COMPOSITIONS FOR DETECTING EARLY STAGE BREAST CANCER WITH RNA-SEQ EXPRESSION PROFILING [P] . 世界知识产权组织专利： WO2018174861A1 . 2018-09-27

  机译： RNA序列表达分析检测早期乳腺癌的方法和组成

• 5. METHODS AND COMPOSITIONS FOR DETECTING EARLY STAGE OVARIAN CANCER WITH RNASEQ EXPRESSION PROFILING [P] . 世界知识产权组织专利： WO2018140049A1 . 2018-08-02

  机译： RNA序列表达分析检测早期卵巢癌的方法和成分

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• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Endothelin-2 Differential Expression in Normal and Early-Stages of Colon Cancer Development [J] . Mariana Bianchi ,Javier Adur ,Maria F. Izaguirre . 癌症治疗（英文） . 2013,第6期
• 4. Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer [J] . Rui-Qing Peng Ya Ding Xing Zhang Yi-Xin Zeng Xiao- Shi Zhang State Key Laboratory of Oncology in South China Biotherapy Center Cancer Center Sun Yat-Sen University 651 Dongfeng R E Guangzhou 510060 Guangdong Province China Ying-Bo Chen Zhi-Wei Zhou State Key Laboratory of Oncology in South China Department of Gastric and Pancreatic Oncology Cancer Center Sun Yat-Sen University 651 Dongfeng R E Guangzhou 510060 Guangdong Province China Rong Zhang State Key Laboratory of Oncology in South China Department of Endoscopy Cancer Center Sun Yat-Sen University 651 Dongfeng R E Guangzhou 510060 Guangdong Province China Xing-Juan Yu Yi-Xin Zeng State Key Laboratory of Oncology in South China Department of Experimental Research Cancer Center Sun Yat-Sen University 651 Dongfeng R E Guangzhou 510060 Guangdong Province China . 世界胃肠病学杂志：英文版 . 2010,第19期
• 5. 肺癌早期诊断的潜在生物标志物LncRNA差异表达谱研究 [C] . 张艳秋 ,ZHANG Yan-qiu ,SUI Jing . 第十二届全国环境与职业医学研究生学术研讨会暨复旦大学博士生论坛公共卫生篇 . -1
• 6. 结肠癌干细胞分选、差异microRNA表达谱检测以及miR-449b-CCND1、E2F3通路在结肠癌干细胞自我更新的机制研究 [A] . 方砚田 . 2014