首页> 外国专利> METHOD FOR EDITING FILAMENTOUS FUNGAL GENOME THROUGH DIRECT INTRODUCTION OF GENOME-EDITING PROTEIN

METHOD FOR EDITING FILAMENTOUS FUNGAL GENOME THROUGH DIRECT INTRODUCTION OF GENOME-EDITING PROTEIN

机译：直接导入基因组编辑蛋白的丝状真菌基因组编辑方法

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摘要

The present invention provides a method for editing a filamentous fungal genome through direct introduction of a genome-editing protein molecule or complex. Said method includes three aspects. According to the first aspect, a genome-editing protein molecule or complex for a target gene is directly introduced into an Aspergillus fungal cell to edit a gene in the Aspergillus fungal genome. According to the second aspect, a desired DNA fragment and a genome-editing protein molecule or complex for a target region in the filamentous fungal genome is directly introduced into a filamentous fungal cell to knock-in the DNA fragment at a desired target site in the filamentous fungal genome. According to the third aspect, genome-editing protein molecules or complexes for multiple target genes are directly introduced into a filamentous fungal cell to co-edit the multiple genes in the filamentous fungal genome.

机译：本发明提供了一种通过直接引入基因组编辑蛋白分子或复合物来编辑丝状真菌基因组的方法。所述方法包括三个方面。根据第一方面，将靶基因的基因组编辑蛋白分子或复合物直接引入曲霉真菌细胞中以编辑曲霉真菌基因组中的基因。根据第二方面，将丝状真菌基因组中靶区域的所需DNA片段和基因组编辑蛋白分子或复合物直接引入丝状真菌细胞中，以将DNA片段敲入到丝状真菌基因组中的所需靶位点。丝状真菌基因组。根据第三方面，将用于多个靶基因的基因组编辑蛋白分子或复合物直接引入丝状真菌细胞中以共同编辑丝状真菌基因组中的多个基因。 展开▼

著录项

公开/公告号WO2018123134A1

专利类型

公开/公告日2018-07-05

原文格式PDF

申请/专利权人 NATIONAL RESEARCH INSTITUTE OF BREWING;FASMAC CO. LTD.;
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申请/专利号WO2017JP30501

发明设计人 IWASHITA KAZUHIRO;ODA KEN;SHIMAMOTO KOHEI;WADA YUSAKU;
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申请日2017-08-25

分类号C12N15/09;C12N1/15;

国家 WO

入库时间 2022-08-21 12:43:29

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3. 马铃薯基因组编辑技术的研究进展及应用前景 [C] . 贺红霞 ,李楠 ,朱旭 . 2018年中国马铃薯大会 . 2018

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