首页> 外国专利> PRODUCTION OF 11Α-HYDROXYLATED STEROIDS BY BIOTRANSFORMATION WITH RECOMBINANT BACTERIA

PRODUCTION OF 11Α-HYDROXYLATED STEROIDS BY BIOTRANSFORMATION WITH RECOMBINANT BACTERIA

机译：通过重组细菌的生物转化生产11α-羟基化甾体

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摘要

The present invention provides a method for producing 11α-hydroxylated steroid compounds or derivatives thereof by fermentation of natural sterols or by biotransformation of various steroidal synthons with recombinant bacteria, preferably Mycobacterium smegmatis or Corynebacteriumglutamicum. Said recombinant bacteria carry a plasmid comprising a synthetic operon containing the genes cyp509C12 and roCPR1 from Rhizopus oryzae, which code for cytochrome CYP509C12 with 11α-hydroxylase activity, and the cytochrome reductase (RoCPR1) needed for activity of the cytochrome. Using the method described in the present invention, 11α OH-ADD or 11α-OH-AD can be produced from natural sterols such as cholesterol or phytosterols, and 1α-OH-PROG, 11α-OH-DOC, 11α-OH-TEST, 11α-OH-DHEA, 11α-OH-AD and/or 11α-OH-ADD can be produced from the corresponding non-hydroxylated synthons, amongst others.

机译：本发明提供了一种通过天然固醇的发酵或通过用重组细菌，优选耻垢分枝杆菌或棒状杆菌对多种甾体合成子的生物转化来生产11α-羟基化甾族化合物或其衍生物的方法。 > 谷氨酸。所述重组细菌携带包含合成操纵子的质粒，所述操纵子包含来自米曲根霉的基因 cyp509C12 和 roCPR1 ，其编码具有11α-羟化酶的细胞色素CYP509C12。活性，以及细胞色素活性所需的细胞色素还原酶（RoCPR1）。使用本发明中描述的方法，可以由天然固醇例如胆固醇或植物固醇以及1α-OH-PROG，11α-OH-DOC，11α-OH-TEST产生11αOH-ADD或11α-OH-AD， 11α-OH-DHEA，11α-OH-AD和/或11α-OH-ADD可以由相应的非羟基化合成子等产生。 展开▼

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公开/公告号WO2017207844A1

专利类型

公开/公告日2017-12-07

原文格式PDF

申请/专利权人 CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC);
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申请/专利号WO2017ES70363

发明设计人 FELPETO SANTERO CARMEN;GALÁN SICILIA BEATRIZ;GARCÍA LÓPEZ JOSÉ LUIS;
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申请日2017-05-29

分类号C12Q1/68;C12N15/77;

国家 WO

入库时间 2022-08-21 12:46:56

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