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Manufacture and use of single-stranded RNA synthesized in vitro for introduction into mammalian cells to induce a biological or biochemical effect
Manufacture and use of single-stranded RNA synthesized in vitro for introduction into mammalian cells to induce a biological or biochemical effect
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机译:体外合成并用于引入哺乳动物细胞以诱导生物或生化作用的单链RNA的制造和使用
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摘要
An ex vivo or in vitro method for obtaining the expression of at least one protein of interest in a cell comprising: contacting a cell with an RNA composition comprising in vitro synthesized RNA encoding at least one protein of interest, in wherein said RNA composition has been treated with RNase III in a buffered aqueous solution comprising magnesium cations at a concentration of about 1-4 mM and a salt that provides an ionic strength equivalent to at least about 50-300 mM acetate of potassium or potassium glutamate, in which less than 0.01% of the mass of the RNA of said treated RNA composition is double stranded RNA greater than about 40 base pairs in length, so that said at least one Protein of interest is expressed in said cell.
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