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A METHOD FOR RAPID 3D PROTOTYPING OF SKELETAL MUSCLE TISSUE CONSTRUCTS USING A NOVEL COMPOSITION OF BIO-INK
A METHOD FOR RAPID 3D PROTOTYPING OF SKELETAL MUSCLE TISSUE CONSTRUCTS USING A NOVEL COMPOSITION OF BIO-INK
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机译：一种利用新型生物墨水组成的骨骼肌组织快速3D原型制作方法
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摘要
The present invention provides the composition of the bio-ink comprising of gelatin, sodium alginate, collagen peptide (Hydrolysed Type I Collagen), secondary mouse muscle myoblast C2C12 cells, and complete growth medium (89% - Dulbecco’s Modified Eagle Medium (DMEM), 10% - Fetal bovine serum and 1% penicillin-streptomycin). Gelatin and sodium alginate are naturally occurring biodegradable biopolymers. Gelatin is thermo-reversibly polymerizable hydrogel and alginate has cross-linking properties which makes the combination of the two, a suitable scaffold for rapid prototyping of constructs having live cells. Addition of CaCl2 provides Ca2+ ions for cross-linking sodium alginate which strengthens the structure of the scaffold thereby providing stability. The bio-ink forms a suitable scaffold with adequate mechanical strength, and also provides an efficient material for growth and differentiation of muscle cells.
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