首页> 外国专利> BRIEF PURIFICATION PROCEDURE FOR THE PREPARATION OF STREPTOCOCCUS PNEUMONIAE-Capsule POLYACCHARIDES

BRIEF PURIFICATION PROCEDURE FOR THE PREPARATION OF STREPTOCOCCUS PNEUMONIAE-Capsule POLYACCHARIDES

机译:肺炎链球菌-荚膜多糖的制备的简单纯化程序

摘要

A shortened process for producing a solution containing substantially purified capsular polysaccharides from a cellular Streptococcus pneumoniae lysate broth is described. Ultrafiltering and diafiltering a clarified S. pneumoniae lysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitated at least 98% of the protein in the solution without seriously affecting polysaccharide yield. Furthermore, following ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitated at least 90% of remaining protein without seriously affecting polysaccharide yield. Exemplary, non-limiting S. pneumoniae serotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. In one embodiment, the Streptococcus pneumoniae cells are lysed using deoxycholate sodium (DOC), while in another embodiment the lytic agent is a non-animal derived lytic agent such as N-lauryl sarcosine sodium (NLS).
机译:描述了一种从细胞肺炎链球菌裂解物肉汤生产含有基本上纯化的荚膜多糖的溶液的简化方法。对澄清的肺炎链球菌裂解物进行超滤和渗滤,然后将pH值调节至小于4.5,最好约为3.5,可使溶液中至少98%的蛋白质沉淀出来,而不会严重影响多糖的得率。此外,在超滤,渗滤和酸化至pH值低于4.5之后,使用活性炭进行的过滤会沉淀出至少90%的残留蛋白质,而不会严重影响多糖的得率。可以使用本发明的缩短的方法纯化的非限制性肺炎链球菌血清型的例子是1、4、5、6A,6B,7F,9V,14、18C,19A,19F和23F。在一个实施方案中,使用脱氧胆酸钠(DOC)裂解肺炎链球菌细胞,而在另一实施方案中,裂解剂是非动物来源的裂解剂,例如N-月桂基肌氨酸钠(NLS)。

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