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首页> 外国专利> CROTALARIA LECTINS SPECTABILIS LEPTOSPIRA BACTERIA AGLUTINATORS POSSIBLE INDICATION AS MARKERS IN FAST DIAGNOSIS OF LEPTOSPIROSIS

CROTALARIA LECTINS SPECTABILIS LEPTOSPIRA BACTERIA AGLUTINATORS POSSIBLE INDICATION AS MARKERS IN FAST DIAGNOSIS OF LEPTOSPIROSIS

机译：猪屎豆胶菌钩端螺旋体增菌剂可能作为快速诊断钩端螺旋体的标志

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摘要

Leptospira bacterial agglutinating lectures of crotalaria spectabilis possible indication as markers in rapid diagnosis of leptospirosis. The present invention relates to the identification and purification for the first time of proteins capable of binding pathogenic leptospires in in vitro assays. Its binding activity on these bacteria occurs even when they are in low concentration, or when lectin has been diluted after protein precipitation. the proteins used in this invention were extracted from crotalaria spectabilis seed, which were precipitated in acetone (80%), lyophilized and isolated by filtration chromatography (sephadex a-100) followed by ion exchange chromatography (deae sephadex a-50). . The unpurified extracts also showed binding capacity on these bacteria. Leptospirosis is an emerging zoonosis with worldwide distribution and is most commonly found in low-income populations in developing countries. Rapid detection of the disease is a critical step in effectively controlling leptospirosis in humans and animals. Thus, there is an urgency to find a fast and efficient diagnosis to identify outbreaks of leptospirosis. Lectins are defined as proteins with the ability to agglutinate red blood cells and recognize different blood types. This property is due to the fact that these molecules recognize and bind to specific sugars on the cell surface. Since leptospires also have sugars on their cell surface, our results indicate that the lectins of crotalaria spectabilis are able to recognize and bind to these bacterial surface sugars, serving as a possible marker in rapid diagnostic tests. The present invention should be supplemented for use as a diagnostic method for leptospirosis. Additional assays of bacterial specificity, protein toxicity and signal amplification will be required. Due to the severity of the disease and the possible lethality of the hosts in a short period of time, a quick diagnosis can prevent death and signal the need for treatment in a short period of time. These tests have been the subject of study in the pharmaceutical and biotechnology industry, but an efficient method that results in the early stages of the disease has not yet been discovered. The present invention addresses the indication of a lectin protein as a possible molecular marker of leptospira bacteria present in the blood of individuals with leptospirosis disease. In addition to humans, complementary tests may indicate the use of these proteins in the diagnosis of disease in dogs and other animals.
机译：钩端螺旋体的钩端螺旋体细菌凝集讲座可能是快速诊断钩端螺旋体病的标志。本发明涉及在体外测定中首次鉴定和纯化能够结合致病性钩端螺旋体的蛋白质。即使它们处于低浓度或凝集素在蛋白质沉淀后被稀释，其对这些细菌的结合活性仍会发生。从猪屎豆种子中提取本发明所用的蛋白质，将其沉淀在丙酮（80％）中，冻干并通过过滤色谱法（sephadex a-100）和离子交换色谱法（deae sephadex a-50）分离。 。未纯化的提取物还显示出对这些细菌的结合能力。钩端螺旋体病是一种新兴的人畜共患病，分布在世界各地，最常见于发展中国家的低收入人群。快速检测疾病是有效控制人和动物钩端螺旋体病的关键步骤。因此，迫切需要找到一种快速有效的诊断方法来鉴定钩端螺旋体病的暴发。凝集素定义为具有凝集红细胞和识别不同血型的能力的蛋白质。该性质是由于这些分子识别并结合细胞表面上的特定糖这一事实所致。由于钩端螺旋体的细胞表面也有糖，因此我们的结果表明，猪屎豆的凝集素能够识别并结合这些细菌表面的糖，在快速诊断测试中可能作为标记。本发明应补充用作钩端螺旋体病的诊断方法。需要进行细菌特异性，蛋白质毒性和信号放大的其他测定。由于疾病的严重性和宿主在短时间内可能的致死性，快速诊断可以预防死亡并在短时间内表明需要治疗。这些测试一直是制药和生物技术行业的研究主题，但尚未发现导致疾病早期的有效方法。本发明解决了凝集素蛋白作为在钩端螺旋体病疾病个体的血液中存在的钩端螺旋体细菌的可能分子标记的指示。除人类外，补充测试还可能表明这些蛋白质在狗和其他动物的疾病诊断中的用途。

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