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NUCLEIC ACID AMPLIFICATION REACTION METHOD, NUCLEIC ACID AMPLIFICATION REACTION REAGENT, AND METHOD OF USING NUCLEIC ACID AMPLIFICATION REACTION REAGENT
NUCLEIC ACID AMPLIFICATION REACTION METHOD, NUCLEIC ACID AMPLIFICATION REACTION REAGENT, AND METHOD OF USING NUCLEIC ACID AMPLIFICATION REACTION REAGENT
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机译：核酸扩增反应试剂，核酸扩增反应试剂以及使用核酸扩增试剂的方法
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摘要
A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a template nucleic acid, a primer, a probe, and a polymerase, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, the calculated Tm value of the primer is 65° C. or higher and 80° C. or lower, a ΔTm value obtained by subtracting the actually measured Tm value of the primer from the actually measured Tm value of the probe is −11° C. or more and 2° C. or less, the calculated Tm value is a value calculated according to a calculation formula, and the actually measured Tm value is an actually measured value obtained by actual measurement.
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