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CONSTRUCTS AND METHODS FOR GENOME EDITING AND GENETIC ENGINEERING OF FUNGI AND PROTISTS

机译:真菌和保护基因的基因编辑和基因工程的结构和方法

摘要

Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
机译:本文提供了用于基因组编辑或对其真菌或原生生物进行基因工程的构建体,使用该构建体的方法和用于选择细胞的培养基。所述构建体包括编码可操作地连接至启动子,合适地为组成型启动子的胸苷激酶的多核苷酸;编码可操作地连接至诱导型启动子的核酸内切酶的多核苷酸;以及核酸内切酶的识别位点。所述构建体还可包括用于选择重组的可选择标记。

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