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METHOD FOR EXAMINING MICROORGANISM, KIT FOR EXAMINING MICROORGANISM, MICROARRAY FOR EXAMINING MICROORGANISM, CARRIER FOR DETECTING FUNGI, METHOD FOR DETECTING FUNGI AND KIT FOR DETECTING FUNGI
METHOD FOR EXAMINING MICROORGANISM, KIT FOR EXAMINING MICROORGANISM, MICROARRAY FOR EXAMINING MICROORGANISM, CARRIER FOR DETECTING FUNGI, METHOD FOR DETECTING FUNGI AND KIT FOR DETECTING FUNGI
A method for detecting microorganisms includes: collecting a sample from a plant tissue, soil, water or any other environment, the sample including microorganisms; extracting DNA from the microorganisms in the sample; conducting PCR using the extracted DNA; and determining the presence or absence of a microorganism belonging to the genus Plasmodiophora in the sample by detecting an obtained amplification product. The PCR is conducted using a first primer set for amplifying a β-tubulin gene of the microorganism belonging to the genus Plasmodiophora. The first primer set includes a forward primer including at least one of base sequences represented by SEQ ID NOS: 1-3 and a reverse primer comprising at least one of base sequences represented by SEQ ID NOS: 4 and 5.
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机译：一种检测微生物的方法，包括：从植物组织，土壤，水或任何其他环境中收集样品，所述样品包括微生物;以及从样品中的微生物中提取DNA;使用提取的DNA进行PCR;通过检测获得的扩增产物，确定样品中是否存在 Plasmodiophora 属微生物。使用第一引物组进行PCR，所述第一引物组用于扩增属于 Plasmodiophora 属的微生物的β-微管蛋白基因。第一引物组包括正向引物和反向引物，正向引物包括SEQ ID NOS：1-3表示的碱基序列中的至少一个，反向引物包括SEQ ID NO：4和5表示的碱基序列中的至少一个。
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