首页> 外国专利> Electron Spin Labeled Ice Binding Compounds Used For Carrying Paramagnetic Centers For Dynamic Nuclear Polarization

Electron Spin Labeled Ice Binding Compounds Used For Carrying Paramagnetic Centers For Dynamic Nuclear Polarization

机译:电子自旋标记的冰结合化合物,用于携带用于动态核极化的顺磁中心

摘要

Spin-labeled ice binding compounds (IBCs) including ice binding proteins (IBPs) or antifreeze proteins (AFPs) and their analogs may carry paramagnetic centers for dynamic nuclear polarization (DNP), for enhancing nuclear magnetic resonance (NMR) signal intensities. Use of spin-labeled IBCs to perform DNP exploits the IBCs' ability to homogeneously distribute the paramagnetic centers in frozen water solution at low temperature, leading to high DNP efficiency. Other advantages of using spin-labeled IBCs include cryo-protecting biological samples; cryo-preserving relative positions and orientations of the spin labeling groups; selecting positions and orientations of spin labeling groups with freedom and without technical barriers to making multiple spin labels in an IBC; and enabling use of a solvent that is primarily water for DNP at low temperatures in view of the potentially high water solubilities of spin-labeled IBCs.
机译:包括冰结合蛋白(IBP)或防冻蛋白(AFP)及其类似物的自旋标记冰结合化合物(IBC)可能带有用于动态核极化(DNP)的顺磁中心,以增强核磁共振(NMR)信号强度。使用自旋标记的中型散货箱进行DNP的开发利用了中型散货箱在低温下将顺磁中心均匀分布在冷冻水溶液中的能力,从而提高了DNP的效率。使用自旋标记的中型散货箱的其他优势包括冷冻保护生物样品;自旋标记基团的冷冻保存的相对位置和方向;自由地选择旋转标记组的位置和方向,并且没有在IBC中制作多个旋转标记的技术障碍;鉴于自旋标记中型散货箱可能具有较高的水溶性,因此能够在低温下使用主要是水的溶剂作为DNP。

著录项

  • 公开/公告号US2018044382A1

    专利类型

  • 公开/公告日2018-02-15

    原文格式PDF

  • 申请/专利权人 THE TRUSTEES OF CALIFORNIA STATE UNIVERSITY;

    申请/专利号US201715652036

  • 发明设计人 YONG BA;YOUGANG MAO;

    申请日2017-07-17

  • 分类号C07K14;A61K49;C07K14/46;A61K49/14;

  • 国家 US

  • 入库时间 2022-08-21 13:04:06

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号