首页> 外国专利> Method for preparing suspension culture-adapted adherent cells, method for inducing epithelial-mesenchymal transition of adherent epithelial cells, and use thereof

Method for preparing suspension culture-adapted adherent cells, method for inducing epithelial-mesenchymal transition of adherent epithelial cells, and use thereof

机译:制备适于悬浮培养的贴壁细胞的方法,诱导贴壁上皮细胞的上皮-间质转化的方法及其用途

摘要

The present invention has a step of culturing adherent cells in contact with an alicyclic structure-containing polymer molded product, a method for preparing adherent cells adapted to suspension culture, and adapted to the suspension culture by this method. Method for producing nucleic acid or protein encoded by foreign gene by culturing adherent cell, suspension culture acclimatization promoter for adherent cell comprising alicyclic structure-containing polymer molding, adherent cell in liquid medium Use of an alicyclic structure-containing polymer molded body for acclimatization to suspension culture, an adhesion-type cell culture container for adhesion type cells, at least the bottom surface comprising an alicyclic structure-containing polymer molded body, A method of inducing epithelial-mesenchymal transition having a step of culturing in a state in contact with an alicyclic structure-containing polymer molded product, by culturing adhesive cells that have undergone epithelial-mesenchymal transition by the above-described induction method, It is encoded nucleic acid or a method of producing the protein such as by coming gene.
机译:本发明具有培养与含有脂环结构的聚合物成型体接触的贴壁细胞的步骤,制备适合于悬浮培养的贴壁细胞的方法,以及通过该方法适合于悬浮培养的贴壁细胞的方法。通过粘附细胞的培养,外来基因编码的核酸或蛋白质的制造方法,包含脂环式结构的聚合物成型体的粘附细胞的悬浮培养驯化促进剂,液体培养基中的粘附细胞,将脂环式结构的聚合物成型体用于悬浮的适应性用途培养,粘附型细胞的粘附型细胞培养容器,其至少底面包含含有脂环式结构的聚合物成型体。诱导上皮-间质转化的方法,具有在与脂环式接触的状态下进行培养的步骤。通过以上述诱导方法培养经历了上皮-间质转化的粘附细胞,从而得到含有结构的聚合物成型体,并通过编码核酸等来编码核酸或制备蛋白质的方法。

著录项

  • 公开/公告号JPWO2017104821A1

    专利类型

  • 公开/公告日2018-10-04

    原文格式PDF

  • 申请/专利权人 日本ゼオン株式会社;

    申请/专利号JP20170556475

  • 发明设计人 平野 孝明;三村 由美子;

    申请日2016-12-16

  • 分类号C12N5/07;C12M1;C12N5/10;C12M3;C12N5/077;

  • 国家 JP

  • 入库时间 2022-08-21 13:08:01

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