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PRESERVATION METHOD OF PLACENTAL TISSUE, AND METHOD OF CULTURING ISOLATED STEM CELLS FROM PLACENTAL TISSUE
PRESERVATION METHOD OF PLACENTAL TISSUE, AND METHOD OF CULTURING ISOLATED STEM CELLS FROM PLACENTAL TISSUE
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机译:胎盘组织的保存方法,以及从胎盘组织培养分离的干细胞的方法
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摘要
PROBLEM TO BE SOLVED: To provide a simple long-term storage method for placental tissue and an effective means of culturing isolated stem cells such as MSC from stored placental tissue.;SOLUTION: A method of preserving placental tissue comprises: a step of slicing placental tissue collected from a living organism so as to have a maximum diameter of 6 mm; a step of soaking the sliced placental tissue in cryoprotectant such that the weight of placental tissue/(weight of placental tissue+weight of cryoprotectant) is 10 to less than 50 wt.%; and a step of cryopreserving the placental tissue in a container with the cryoprotectant in an environment at -80°C or less. Further, a method for preparing stem cells derived from frozen placenta comprises: a step of washing the preserved placental tissue with a culture liquid after thawing; a step of washing with a protease solution; and a step of digesting the placental tissue in the protease solution. Further provided are a method for culturing stem cells from the prepared placental tissue, and a method in which the stem cells are mesenchymal cells.;SELECTED DRAWING: None;COPYRIGHT: (C)2018,JPO&INPIT
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