首页> 外国专利> PRESERVATION METHOD OF PLACENTAL TISSUE, AND METHOD OF CULTURING ISOLATED STEM CELLS FROM PLACENTAL TISSUE

PRESERVATION METHOD OF PLACENTAL TISSUE, AND METHOD OF CULTURING ISOLATED STEM CELLS FROM PLACENTAL TISSUE

机译:胎盘组织的保存方法,以及从胎盘组织培养分离的干细胞的方法

摘要

PROBLEM TO BE SOLVED: To provide a simple long-term storage method for placental tissue and an effective means of culturing isolated stem cells such as MSC from stored placental tissue.;SOLUTION: A method of preserving placental tissue comprises: a step of slicing placental tissue collected from a living organism so as to have a maximum diameter of 6 mm; a step of soaking the sliced placental tissue in cryoprotectant such that the weight of placental tissue/(weight of placental tissue+weight of cryoprotectant) is 10 to less than 50 wt.%; and a step of cryopreserving the placental tissue in a container with the cryoprotectant in an environment at -80°C or less. Further, a method for preparing stem cells derived from frozen placenta comprises: a step of washing the preserved placental tissue with a culture liquid after thawing; a step of washing with a protease solution; and a step of digesting the placental tissue in the protease solution. Further provided are a method for culturing stem cells from the prepared placental tissue, and a method in which the stem cells are mesenchymal cells.;SELECTED DRAWING: None;COPYRIGHT: (C)2018,JPO&INPIT
机译:要解决的问题:为胎盘组织提供一种简单的长期保存方法,以及从保存的胎盘组织中培养分离的干细胞(如MSC)的有效方法。解决方案:一种保存胎盘组织的方法包括:切片胎盘的步骤从活生物体收集的组织,最大直径为6毫米;将切片的胎盘组织浸入冷冻保护剂中的步骤,使得胎盘组织的重量/(胎盘组织的重量+冷冻保护剂的重量)为10至小于50重量%;以及将胎盘组织与冷冻保护剂一起在-80℃以下的环境中冷冻保存的步骤。此外,一种制备源自冷冻胎盘的干细胞的方法包括:在融化后用培养液洗涤保存的胎盘组织的步骤;用蛋白酶溶液洗涤的步骤;消化蛋白酶溶液中的胎盘组织的步骤。还提供了一种从制备的胎盘组织中培养干细胞的方法,以及一种以干细胞为间充质细胞的方法。选图:无;版权:(C)2018,JPO&INPIT

著录项

  • 公开/公告号JP2018093782A

    专利类型

  • 公开/公告日2018-06-21

    原文格式PDF

  • 申请/专利权人 TOYOBO CO LTD;

    申请/专利号JP20160240992

  • 发明设计人 YAMAGUCHI TATSUYA;

    申请日2016-12-13

  • 分类号C12N5/071;C12N5/0775;A61K35/50;C12M3/00;

  • 国家 JP

  • 入库时间 2022-08-21 13:13:24

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