首页> 外国专利> METHOD FOR MAKING THREE-DIMENSIONAL CULTURED SKIN MODEL INCLUDING DERMIS AND EPIDERMIS, AND THREE-DIMENSIONAL CULTURED SKIN MODEL MADE THEREBY

METHOD FOR MAKING THREE-DIMENSIONAL CULTURED SKIN MODEL INCLUDING DERMIS AND EPIDERMIS, AND THREE-DIMENSIONAL CULTURED SKIN MODEL MADE THEREBY

机译:包括真皮和表皮的三维培养皮肤模型的制作方法,从而制作了三维培养皮肤模型

摘要

The present invention relates to a method for preparing a three-dimensionally cultured skin model comprising dermis and epidermis, which comprises: a step of preparing the dermis using a composition comprising murine fibroblasts; and native collagen or a combination of native collagen and atelocollagen; and a step of forming the epidermis using keratinocytes. Also, the present invention relates to a three-dimensionally cultured skin model which comprises: a dermis prepared by a composition comprising murine fibroblasts, native collagen, or a combination of native collagen and atelocollagen; and epidermis formed from keratinocytes. The three-dimensionally cultured skin model of the present invention may be used widely in toxicity and efficacy experiments of medicines or cosmetics, and in the field of alternative experiments for animal experiments since the three-dimensionally cultured skin model is excellent in formation and differentiation of dermis and epidermis using murine 3T3 cells for preparing the skin model and a mixture of atelocollagen and native collagen, and has a structure similar to the human skin layer by inhibiting dermal contraction and collagen degradation in the dermis.
机译:本发明涉及一种制备包含真皮和表皮的三维培养皮肤模型的方法,该方法包括:使用包含鼠成纤维细胞的组合物制备真皮的步骤;天然胶原蛋白或天然胶原蛋白与胶原蛋白的组合;以及使用角质形成细胞形成表皮的步骤。另外,本发明涉及三维培养的皮肤模型,其包括:通过包含鼠成纤维细胞,天然胶原蛋白或天然胶原蛋白与胶原蛋白的组合物的组合物制备的真皮;和和由角质形成细胞形成的表皮。本发明的三维培养的皮肤模型可广泛用于药物或化妆品的毒性和功效实验,以及动物实验的替代实验领域,因为三维培养的皮肤模型在形成和分化方面极好。真皮和表皮使用鼠类3T3细胞制备皮肤模型以及端胶原和天然胶原蛋白的混合物,并通过抑制真皮中的真皮收缩和胶原蛋白降解而具有类似于人皮肤层的结构。

著录项

  • 公开/公告号EP3167913A4

    专利类型

  • 公开/公告日2018-03-07

    原文格式PDF

  • 申请/专利权人 JEON SAEWHA;

    申请/专利号EP20150819565

  • 发明设计人 KIM YUN HEE;MOON HYUN AH;

    申请日2015-07-09

  • 分类号A61L27/60;A61L27/38;A61L27/24;

  • 国家 EP

  • 入库时间 2022-08-21 13:16:59

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