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METHOD FOR EVALUATING MESENCHYMAL STEM CELL ACTIVITY, METHOD FOR CULTURING MESENCHYMAL STEM CELLS, METHOD FOR PRODUCING THERAPEUTIC AGENT FOR LIVER DYSFUNCTION, AND THERAPEUTIC AGENT FOR LIVER DYSFUNCTION
METHOD FOR EVALUATING MESENCHYMAL STEM CELL ACTIVITY, METHOD FOR CULTURING MESENCHYMAL STEM CELLS, METHOD FOR PRODUCING THERAPEUTIC AGENT FOR LIVER DYSFUNCTION, AND THERAPEUTIC AGENT FOR LIVER DYSFUNCTION
The present invention relates to a method for evaluating the activity level of mesenchymal stem cells, and a method for culturing mesenchymal stem cells using the evaluation method in the field of culturing mesenchymal stem cells for regenerative medicine, and further, a method for producing a therapeutic agent for liver dysfunction and a therapeutic agent for liver dysfunction. This method for evaluating mesenchymal stem cell activity according to the present invention comprises an assay step for assaying the amount of adenylate kinase 4 (AK4) in the mesenchymal stem cells; and a determination step for determining the activity level of the mesenchymal stem cells from the assayed amount of adenylate kinase 4. In this method, the activity level of mesenchymal stem cells can be evaluated from the assayed amount of AK4, for instance, if the mitochondrial activity level of mesenchymal stem cells is high, the mesenchymal stem cells can be evaluated as ideal for use as a therapeutic agent, while if mitochondrial activity of the mesenchymal stem cells is low and aging does not proceed, the mesenchymal stem cells can be evaluated as suitable for subculture.
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