首页> 外国专利> E. coli BL21 (DE3)/pTEV-TMS STRAIN - FUSION PROTEIN TrxTEVrs-TMS PRODUCER, DESTINED FOR PROTEOLYTIC CLEAVAGE WITH TUMSTATIN ANTI-ANGIOGENIC PEPTIDE FORMATION, HUMAN TUMSTATIN FRAGMENT L69K-95 DERIVATIVE, AND METHOD FOR RECOMBINANT ANTI-ANGIOGENIC PEPTIDE PRODUCTION

E. coli BL21 (DE3)/pTEV-TMS STRAIN - FUSION PROTEIN TrxTEVrs-TMS PRODUCER, DESTINED FOR PROTEOLYTIC CLEAVAGE WITH TUMSTATIN ANTI-ANGIOGENIC PEPTIDE FORMATION, HUMAN TUMSTATIN FRAGMENT L69K-95 DERIVATIVE, AND METHOD FOR RECOMBINANT ANTI-ANGIOGENIC PEPTIDE PRODUCTION

机译：大肠杆菌BL21（DE3）/ pTEV-TMS菌株-融合蛋白TrxTEVrs-TMS生产商，旨在通过肠胃抑素抗血管肽的形成，人胃抑素片段[L69K-95]变性胶粘剂和方法用于蛋白水解生产

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FIELD: biotechnology.;SUBSTANCE: method for recombinant anti-angiogenic Tumastin peptide a - human tumstatin modified fragment [L69K-95] derivative with Ser-Gly-Ala-Met-Gly and Pro-Gly-Pro sequences attached to N- and C-termini, respectively, provides culturing of E. coli BL21 (DE3)/pTEV-TMS producing strain, destruction of cell biomass in the presence of protease inhibitors, cell lysate centrifuging and separation of cell supernatant containing fusion protein, fusion protein chromatographic purification on a column with an anion exchange sorbent, proteolytic cleavage by TEV-protease, chromatographic purification of the anti-angiogenic Tumastin peptide.;EFFECT: invention provides high yield of anti-angiogenic Tumastin peptide.;2 cl, 5 dwg, 3 ex

机译：领域：生物技术;方法：重组抗血管生成性Tumastin肽a-人tumstatin修饰的片段[L69K-95]衍生物，其Ser-Gly-Ala-Met-Gly和Pro-Gly-Pro序列附着于N-和C -termini分别提供大肠杆菌BL21（DE3）/ pTEV-TMS产生菌株的培养，蛋白酶抑制剂存在下细胞生物量的破坏，细胞裂解液的离心和含融合蛋白的细胞上清液的分离，融合蛋白的色谱纯化具有阴离子交换吸附剂的柱，通过TEV蛋白酶进行蛋白水解切割，色谱纯化抗血管生成的Tumastin肽。效果：本发明提供了高产率的抗血管生成的Tumastin肽。2cl，5 dwg，3 ex

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公开/公告号RU2625008C2

专利类型
公开/公告日2017-07-11

原文格式PDF
申请/专利权人 FEDERALNOE GOSUDARSTVENNOE BYUDZHETNOE UCHREZHDENIE NAUKI INSTITUT BIOORGANICHESKOJ KHIMII IM. AKADEMIKOV M.M. SHEMYAKINA I YU.A. OVCHINNIKOVA ROSSIJSKOJ AKADEMII NAUK (IBKH RAN);
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申请/专利号RU20150147716
发明设计人 ESIPOV ROMAN STANISLAVOVICH (RU);KOSTROMINA MARIYA ANDREEVNA (RU);BEJRAKHOVA KSENIYA ANDREEVNA (RU);MAKAROV DMITRIJ ALEKSANDROVICH (RU);MIROSHNIKOV ANATOLIJ IVANOVICH (RU);
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申请日2015-11-06
分类号C12N15/62;C12N15/70;C12N15/24;C12N1/21;C12P21/02;C07K1/16;C12R1/19;
国家 RU
入库时间 2022-08-21 13:23:29

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